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Bordetella avium is a gram-negative, non-fermentative, aerobic and motile bacteria from the family Alcaligenaceae. Bordetella avium mainly infects young, domesticated turkeys, it can also infect quail and ostrich chicks. In cockatiels, B. avium is known to cause lockjaw syndrome. Other respiratory symptoms caused by B. avium incudes sneezing, ocular and nasal discharge, inflammation, etc. The disease caused by B. avium in birds is called bordetellosis. Avian bordetellosis is a highly infectious, acute upper respiratory tract disease of turkeys characterized by high morbidity and usually low mortality. Bordetellosis has been identified in almost every area of the world where turkeys are intensively reared. The economic impact of bordetellosis is enormous because impaired growth and mortality resulting from secondary colisepticemia cause huge losses annually to the turkey industry around the world.
Fig 1. SEM of tracheae incubated with B. avium (Temple, et al. 1998).
Bordetella avium PCR Kit Development
Bordetella avium has major impact on turkey health within turkey production systems. Good management practices are essential for controlling disease caused by B. avium. Despite the increasing use and superior performance of PCR-based diagnostic assays in clinical laboratories, the identification of B. avium still relies on isolation and biochemical assays. The slow growth of B. avium also complicates this approach. Numerous polymerase chain reactions (PCR) have been developed for the identification of B. avium, such as real-time PCR (TaqMan) analysis, multiplex PCR, and single-duplex PCR. Among them, real-time PCR (TaqMan) analysis is the most popular and provides high specificity and efficiency.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of Bordetella avium according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Bordetella avium PCR Kit We Can Develop
| Bordetella avium TaqMan real-time PCR kit | Bordetella avium multiplex RT-PCR kit |
| Bordetella avium RT-PCR kit | Bordetella avium singleplex RT-PCR kit |
Workflow of Bordetella avium PCR Kit Development
| Workflow | Details |
| Bacterial DNA extraction | Extract the bacterial DNA from the Bordetella avium-positive sample. |
| Screening | Screening for Bordetella avium by TaqMan real-time PCR. |
| Designing of specific primers | Special primers can be designed using professional software and database. |
| Applying thermal cycling parameters | Apply specific temperature (two-stage temperature profile) for a specific period of time. |
| Assessment of TaqMan RT-PCR specificity | Evaluate the test specificity by testing DNA isolated from other pathogens. |
| Evaluation of TaqMan RT-PCR sensitivity | Evaluate the assay sensitivity by preparing a standard curve. |
Delivery
- A series of PCR kits for Bordetella avium
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Compatible with a large number of samples
- Reasonable price and short turnaround time
References
- Temple, Louise M., et al. "Bordetella avium virulence measured in vivo and in vitro." Infection and Immunity 66.11 (1998): 5244-5251.
- Arp, L. H., R. D. Leyh, and R. W. Griffith. "Adherence of Bordetella avium to tracheal mucosa of turkeys: correlation with hemagglutination." American Journal of Veterinary Research 49.5 (1988): 693-696.
- Hellwig, D. H., L. H. Arp, and J. A. Fagerland. "A comparison of outer membrane proteins and surface characteristics of adhesive and non-adhesive phenotypes of Bordetella avium." Avian Diseases (1988): 787-792.
