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The equine gastrointestinal tract includes all the organs that are involved in taking in and processing food. It begins with the mouth and includes the esophagus, stomach, liver, pancreas, intestines, rectum, and anus. The signs of equine gastrointestinal disorders can include excessive drooling, diarrhea, constipation, bleeding, loss of appetite, bloating, dehydration. This can be caused by bacteria (Clostridium difficile, Clostridium perfringens, Neorickettsia risticii), viruses (equine specific coronavirus), and parasites such as strongyles. Equine gastrointestinal disease is an important factor affecting the health of horses and causing losses in horse breeding globally every year. Therefore, research into equine gastrointestinal pathogens is playing more and more significant role in the early diagnosis and prevention of equine gastrointestinal tract diseases.
Figure 1. A model for gut microbiota and their effects on host physiology after natural strongyle infection (Clark et al., 2018).
Equine Gastrointestinal Pathogens AGID Kit Development
Detection and elimination of the pathogens should be a high priority to efficiently control the spread of equine gastrointestinal diseases and reduce the consequent economic losses. The agar gel immunodiffusion (AGID) test is a widely utilized serological technique, which is used to detect group-specific antibodies against certain pathogens. Compared with molecular biological methods, AGID is a simple and convenient method in veterinary diagnosis. Hence, it has been a useful tool for the detection of equine gastrointestinal pathogens in the laboratory. Several AGID techniques have been developed, including radial immunodiffusion enzyme assay, single immunodiffusion assay, double immunodiffusion assay, etc.
BioVenic is a supplier of high-quality animal diseases diagnostic reagents and kits. We have rich professional knowledge and rich experience in the development and optimization of agar gel immunodiffusion kits. According to your specifications, we can develop a variety of customized agar gel immunodiffusion kits for the identification of equine gastrointestinal pathogens. If you have any needs, please feel free to contact us. We will provide you with high-quality products and one-stop services.
Equine Gastrointestinal Pathogens AGID Kit We Can Develop
| C. difficile radial immunodiffusion enzyme assay kit | C. difficile single immunodiffusion assay kit | C. difficile double immunodiffusion assay kit |
| C.perfringens radial immunodiffusion enzyme assay kit | C.perfringens single immunodiffusion assay kit | C.perfringens double immunodiffusion assay kit |
| P. equorum radial immunodiffusion enzyme assay kit | P. equorum single immunodiffusion assay kit | P. equorum double immunodiffusion assay kit |
| Other AGID kits you need |
Workflow of Equine Gastrointestinal Pathogens AGID Kit Development
| Workflow | Details |
| Serum sample collection | Blood was collected from horses and left to clot at a specific temperature. |
| Gel preparation | Agarose gel was prepared in a specific buffered saline solution at a specific temperature for a given period. |
| Control measures | The control experiment was carried to allow the gel to diffuse under the series of temperature for a specific period. |
| Coomassie blue staining | The gel was dried and placed in Coomassie Brilliant Blue |
| Silver staining | Silver staining kit was utilized for further process. |
| Efficiency evaluation | The efficiency of the assay was measured using different analysis. |
Delivery
- A series of AGID kits for equine gastrointestinal pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High test-to-test repeatability
- Detects very minute quantity of equine gastrointestinal pathogens with high sensitivity
- Detection of therapeutic pathogens to a level of higher specificity
- Reasonable price and short turnaround time
- Combine AGID with other detection methods to avoid false positives
References
- Clark, Allison, et al. "Strongyle infection and gut microbiota: profiling of resistant and susceptible horses over a grazing season." Frontiers in Physiology 9 (2018): 272.
- Andersen, U. V., et al. "Recent advances in diagnosing pathogenic equine gastrointestinal helminths: the challenge of prepatent detection." Veterinary Parasitology 192.1-3 (2013): 1-9.
- Bland, Stephanie D. "Equine colic: a review of the equine hindgut and colic." Veterinary Science Development 6.1 (2016).
