Services
Pseudorabies virus (PRV), also known as porcine herpesvirus type I, belongs to the subfamily Alphaherpesvirinae of the family Herpesviridae. Pseudorabies virus particles are oval or round in shape, mature virus particles with envelope are about 150-180 nm in diameter, and the surface of the envelope has radially arranged spikes with a length of about 8-10 nm. The genome of PRV is a linear double-stranded DNA molecule of about 150 kb that encodes more than 100 proteins. Among the main structural proteins of PRV, the membrane proteins gB and gC mainly stimulate the immune response, and the gE protein is one of the main virulence factors of PRV. The genes encoding these three proteins are widely used to monitor the genetic evolution of PRV.
Figure 1. Structure of the PRV virion. PRV virions are composed of four structural elements. The double-stranded DNA genome is housed in an icosahedral capsid. The tegument is a collection of approximately 12 proteins organized into at least two layers, one which interacts with envelope proteins and one that is closely associated with the capsid. The envelope is a lipid bilayer infused with transmembrane proteins, many of which are modified by glycosylation (Pomeranz, Lisa E., et al. 2005).
Pseudorabies Virus Isolation Kit Development
PRV is the primary pathogenic factor of pseudorabies, which can infect pigs, cattle, sheep, cats and dogs, but cannot infect humans and primates. Porcine pseudorabies has fever, itching and encephalomyelitis as the main clinical features, among which the neurological symptoms of piglets are more obvious, and the fatality rate can reach 100%. The spread of pseudorabies virus has brought huge economic losses to the pig industry. Virus isolation, as the gold standard for the etiological diagnosis of viral diseases, is used for the isolation and identification of pseudorabies virus.
BioVenic is a leading supplier of animal virus diagnostics, dedicated to improving the health of poultry, livestock, companion animals and wildlife. We have advanced virus isolation equipment and our researchers are professionally trained. We can provide you with a series of pseudorabies virus isolation kits to study the pathogenic mechanism and transmission route of pseudorabies virus, and discover the control and treatment methods of pseudorabies. All our kits have undergone strict quality control to ensure more accurate experimental results. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
The Pseudorabies Virus Isolation Kits We Can Develop
Porcine pseudorabies virus isolation kit
Canine pseudorabies virus isolation kit
Feline pseudorabies virus isolation kit
Mouse pseudorabies virus isolation kit
Bovine pseudorabies virus isolation kit
Sheep pseudorabies virus isolation kit
Rabbit pseudorabies virus isolation kit
Other pseudorabies virus isolation kit
Workflow of Pseudorabies Virus Isolation Kit Development
| Workflow | Details |
|---|---|
| Sample collection | Samples are collected from infected animals. |
| Cell culture | Samples were inoculated onto the culture medium for propagation. |
| RNA isolation and purification | Isolation and purification of porcine circovirus RNA from cell culture fluid. |
| Structural analysis of pseudorabies virus | The isolated pseudorabies virus acid and protein coat were analyzed. |
| Pseudorabies virus isolation kit development | Development of kit to isolate pseudorabies virus. |
| Pseudorabies virus isolation kit delivery | We will provide you with customized pseudorabies virus isolation kits and complete product quality inspection reports. |
Delivery
- A series of pseudorabies virus isolation kits
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- Accurate and reliable results
- Advanced equipment and professional technicians
- Reasonable price and short turnaround time
Reference
- Pomeranz, Lisa E., Ashley E. Reynolds, and Christoph J. Hengartner. "Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine." Microbiology and Molecular Biology Reviews 69.3 (2005): 462-500.
