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Newcastle disease virus (NDV) is a single-stranded, non-segmented RNA virus belonging to the family Paramyxoviridae. The NDV genome contains six major genes, which can encode six structural and two nonstructural proteins. The host range of NDV is wide. It is reported that 27 of 50 species of birds can be infected with NDV. Newcastle disease (ND) is a poultry disease caused by NDV with high mortality and morbidity causing considerable economic losses to the world poultry industry.
Figure 1. Structural features of Newcastle disease virus (Bello, et al. 2018).
Newcastle Disease Virus ELISA Kit Development
Currently, a variety of serological assays have been developed for NDV diagnosis. The hemagglutination inhibition (HI) is considered the standard laboratory test for Newcastle disease and is the most widely used serological method for measuring anti-NDV antibodies in poultry serum. However, when serum samples are contaminated, higher false-positive results are generated and the sensitivity of the method is lower. Enzyme-linked immunosorbent assay (ELISA) has the advantages of easy standardization, high-throughput detection, high sensitivity and no need for serum pretreatment. It is an effective tool for NDV detection and can meet the requirements of epidemic prevention.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare specific antibodies against the antigens of Newcastle disease virus and can provide you with a series of customized ELISA kits for the detection of Newcastle disease virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| Newcastle disease virus sandwich ELISA kit | Newcastle disease virus competitive ELISA kit |
| Newcastle disease virus indirect ELISA kit | Newcastle disease virus direct ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
|---|---|
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | The Newcastle disease virus antigens were comprehensively analyzed and evaluated to determine the expression intervals of the antigens, and then recombinant antigens with a purity of not less than 90% were prepared for immunization. |
| Antibody preparation | Preparation of antibodies specific for Newcastle disease virus antigens. |
| ELISA kit development | Antibody label, antibody paired screening, method development and optimization, kit production. |
| ELISA kit delivery | We will provide you with customized ELISA kits and complete product quality inspection report. |
Delivery
- A series of ELISA kits for detection Newcastle disease virus
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Good adsorption performance, low blank value
- Professional antibody platform to support ELISA kit development
- Reasonable price and short turnaround time
References
- Bello, Muhammad Bashir, et al. "Diagnostic and vaccination approaches for Newcastle disease virus in poultry: The current and emerging perspectives." BioMed Research International 2018 (2018).
- Phan, L. V., et al. "Development and field application of a competitive enzyme-linked immunosorbent assay for detection of Newcastle disease virus antibodies in chickens and ducks." Poultry Science 92.8 (2013): 2034-2043.
