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Agar gel immunodiffusion (AGID) test is a precipitation reaction of soluble antigens and antibodies in agar gels. If the antigen and antibody correspond, and the ratio is appropriate, a white precipitation line can be formed at the meeting point, which is a positive reaction. The precipitation line is a group of specific complexes of antigen and antibody. When there are several different groups of antigens and antibodies in agar, each of them will form independent precipitation lines at appropriate sites according to the difference of their diffusion rate. Agar gel immunodiffusion test has two basic types: single immunodiffusion test and double immunodiffusion test.
Figure 1. Standards used for reporting agar gel immunodiffusion test (AGIDT) reactions and challenges with accurate interpretations in the AGIDT: (a) graphical representation of standardised numerical interpretations by intensity and (b) photographs of reactions of weak positive reference serums from United States Department of Agriculture (USDA) (Panel 1) and University of Kentucky (Panel 2) (Issel, et al., 2013).
AGID Kit Development
With the spread of various infectious animal diseases all over the world, it has brought a huge impact on the social economy. In order to control the epidemic of infectious animal diseases, it is necessary to develop a rapid and high-throughput screening method for animal diseases. AGID is a simple and economical serological test with high specificity and sensitivity, it is not only used for the diagnosis of animal diseases, but also for the analysis of antigenic components in a wide range of applications.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal viruses for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. We have a professional team with extensive experience in veterinary diagnostics and kit development, and we can customize a range of AGID kits for animal pathogen detection based on your needs. If you have any needs, please feel free to contact us, we can provide you with high-quality products and services.
The Workflow of AGID Kit Development
There are many things to consider when developing an AGID kit. We can design different AGID kits for you to capture and detect the target antigen depending on your detection target. The following is the workflow of AGID kit development.
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Avian Respiratory Pathogens AGID Kit Development
Equine Respiratory Pathogens AGID Kit Development
Equine Gastrointestinal Pathogens AGID Kit Development
Bovine Respiratory Pathogens AGID Kit Development
Bovine Gastrointestinal Pathogens AGID Kit Development
Porcine Respiratory Pathogens AGID Kit Development
Porcine Gastrointestinal Pathogens AGID Kit Development
Canine Respiratory Pathogens AGID Kit Development
Canine Gastrointestinal Pathogens AGID Kit Development
Feline Respiratory Pathogens AGID Kit Development
Feline Gastrointestinal Pathogens AGID Kit Development
Sheep Respiratory Pathogens AGID Kit Development
Sheep Gastrointestinal Pathogens AGID Kit Development
Ruminant Abortion AGID Kit Development
References
- Issel, C. J., et al. "Challenges and proposed solutions for more accurate serological diagnosis of equine infectious anaemia." Veterinary Record 172.8 (2013): 210-210.
- Jenson, Terra A. "Agar gel immunodiffusion assay to detect antibodies to Type A influenza virus." Animal Influenza Virus. Humana Press, New York, NY, 2014. 141-150.
