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Feline panleukopenia virus (FPV; synonymous with feline parvovirus) is a single-stranded linear DNA virus of the family Parvoviridae. The FPV genome is generally composed of 120-160 bp bases and has hairpin structures at both ends. FPV has a wide range of hosts and can infect a variety of animals such as cats, raccoons and mustelids under natural conditions, but smaller cats and minks are the most susceptible. Feline panleukopenia (FP) is an acute, fatal infectious disease caused by FPV. FPV-infected cats develop severe hemorrhagic gastroenteritis and leukopenia, with mortality ranging from 25% to 90%. Outbreaks of FP may lead to high mortality in cats, affect human health and cause huge economic losses. Therefore, accurate and rapid diagnosis of FPV infection is crucial for the management and treatment of FP.
Figure 1. Feline panleukopenia virus.
Feline Panleukopenia Virus PCR Kit Development
FPV is still widespread worldwide, which has made the development of rapid and immediate diagnostic tools a major global focus in recent decades. Currently, various methods for the diagnosis of FPV infection have been reported, including electron microscopy, immunochromatographic testing, virus isolation, latex agglutination, ELISA, and PCR. Although various assays are suitable for the diagnosis of FPV infection, the process of virus isolation is complicated, electron microscopy requires tedious operation by specialized technicians, and the low sensitivity of antigen and antibody detection is unable to diagnose FPV infection in its early stages. As a rapid, high-sensitivity and high-specificity detection method, PCR is widely used in the diagnosis of FPV, and commonly used PCR methods include conventional PCR, real-time PCR, nested PCR, iiPCR, multiplex PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of feline panleukopenia virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Feline Panleukopenia Virus PCR Kits We Can Develop
| FPV conventional PCR kit | FPV mPCR kit | FPV qPCR kit |
| FPV real-time PCR kit | FPV TaqMan real-time PCR kit | Other FPV PCR kits |
Workflow of Feline Panleukopenia Virus PCR Kit Development
| Workflow | Details |
| Specimen preparation | Specimens can be collected from cats that were infected by FPV. |
| Primer and probes design | Available sequences of FPV can be retrieved from GenBank and specific primer/probe sets were designed. |
| Nucleic acid extraction | Extract DNA from cell cultures, and DNA concentration, purity and integrity were measured. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of DNA from FPV. The sensitivity of PCR kit was assessed by determine the limit of detection (LoD) at the DNA levels. |
Delivery
- A series of PCR kits for feline panleukopenia virus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- High sensitivity to detect very small amounts of feline panleukopenia virus in your samples
- Excellent specificity for the detection of feline panleukopenia virus
- Reasonable price and short turnaround time
References
- Streck, André Felipe, et al. "An updated TaqMan real-time PCR for canine and feline parvoviruses." Journal of Virological Methods 193.1 (2013): 6-8.
- Zhang, Qian, et al. "Development and application of a multiplex PCR method for the simultaneous detection and differentiation of feline panleukopenia virus, feline bocavirus, and feline astrovirus." Archives of Virology 164.11 (2019): 2761-2768.
