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The canine gastrointestinal microbiome plays an important role in the host's nutritional, immune and physiological processes. However, gastrointestinal microbiota dysbiosis is common in canine gastrointestinal diseases such as chronic diarrhea and inflammatory enteropathy. Signs of canine gastrointestinal diseases can be excessive drooling, diarrhea, constipation, vomiting or regurgitation, loss of appetite, bleeding, abdominal pain and bloating, straining to defecate, shock, and dehydration. The main pathogens causing these diseases have been identified, including various Clostridium, Campylobacter, Salmonella, and Escherichia coli. Some viruses have also been found associated with Canine gastrointestinal diseases, such as Caliciviridae, Picornaviridae, Parvoviridae and Circoviridae. The location and nature of the gastrointestinal disease can be determined by the signs the dog shows. However, in order to diagnose the disease more accurately, the detection of canine gastrointestinal pathogens is essential.
Figure 1. The role of domestication in altering the canine gut microbiota (Huang et al., 2020).
Canine Gastrointestinal Pathogens AGID Kit Development
As new research into the diagnosis and control of canine gastrointestinal is developed, veterinarians are continuing to develop novel creative strategies to understand the disease process, control its spread, and minimize its effect. Serology by agar gel immunodiffusion (AGID) is a frequently used method for diagnosing canine gastrointestinal diseases caused by a variety of pathogens. The agar gel immunodiffusion assay is a diagnostic test that employs serum to detect antibodies produced in response to infection. Apart from simplicity, rapidity and low cost, another advantage of the agar gel immunodiffusion test is that it can reliably analyze severely hemolyzed and cytotoxic serum samples. Several AGID technologies have been developed by researchers for the detection of canine gastrointestinal pathogens, such as radial immunodiffusion assay (RID), double immunodiffusion assay, etc.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal pathogens for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. We have a professional and passionate team with considerable veterinary diagnostic and reagent development experience. Based on an advanced biotechnology platform, we can provide a variety of customized agar gel immunodiffusion kits for the detection of canine gastrointestinal pathogens. If you have any questions, please do not hesitate to contact us. We will provide you with high-quality products and services.
Canine Gastrointestinal Pathogens AGID Kits We Can Develop
| Escherichia coli radial immunodiffusion enzyme assay kit | Escherichia coli double immunodiffusion assay kit | Campylobacter radial immunodiffusion enzyme assay kit |
| Campylobacter double immunodiffusion assay kit | Caliciviridae radial immunodiffusion assay kit | Caliciviridae double immunodiffusion assay kit |
| Picornaviridae double immunodiffusion assay kit | Picornaviridae radial immunodiffusion assay kit | Other AGID kits you need |
Workflow of Canine Gastrointestinal Pathogens AGID Kit Development
| Workflow | Details |
| Preparation of agarose gel | Agarose gel was prepared and heated for a given period. |
| Preparation of reference antigen | Reference antigens were taken from the dogs infected with gastrointestinal pathogens. |
| Preparation of antiserum | Antiserum was prepared by collecting the serum from dogs and serum was examined rigorously for specificity. |
| Examination of sample | Samples were diluted using distilled water and conducted for examination. |
| Pathogenic antigen separation | Antigens of pathogens were separated using centrifugation. |
| Interpretation of test results for antibody detection | Interpret the result via different analysis methods and evaluate the efficiency of the assay. |
Delivery
- A set of AGID kits for canine gastrointestinal pathogens
- Report on product quality
- List of other experimental data you need
Our Advantages
- Short turnaround time and reasonable price
- Detects a small number of canine gastrointestinal infections in a high-sensitivity manner
- Detecting therapeutic pathogens with a higher level of specificity
- Combine AGID with other detection methods to avoid false positives
References
- Huang, Zongyu, et al. "The canine gastrointestinal microbiota: early studies and research frontiers." Gut Microbes 11.4 (2020): 635-654.
- Caddy, Sarah L. "New viruses associated with canine gastroenteritis." The Veterinary Journal 232 (2018): 57-64.
- de Oliveira, Ana Laura Bello, et al. "Detection of Brucella spp. in dogs at Pantanal wetlands." Brazilian Journal of Microbiology 50.1 (2019): 307-312.
