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For a long time, various horse pathogens have been important factors leading to horse diseases and adverse effects on the horse breeding industry. The most notable equine diseases that threaten the health of horses include influenza, equine encephalomyelitis, tetanus, equine rabies, equine protozoal myeloencephalitis, contagious equine metritis, and piroplasmosis. A variety of pathogens are involved in equine diseases, such as equine herpesvirus, equine arteritis virus, Venezuelan encephalitis virus, Streptococcus equi and Streptococcus zooepidemicus. The horse industry is unique because horses are transported more frequently and farther than other livestock, the risk of transmission of equine infectious diseases is greatly increased. To prevent the spread of any equine infectious disease, movement restriction, management procedures, hygiene, and disinfection are essential.
Figure 1. Transmission cycle of equid alphaherpesvirus 3 (EHV-3) in mares and stallions (Vissani, et al., 2021).
Equine Pathogens IFA Kit Development
To effectively prevent and control equine diseases, a plan of action based on the evaluations of risk assessment, resource management, and horse management should be settled to manage equine infectious disease outbreaks before the onset of clinical diseases. Therefore, a rapid and accurate identification of the pathogens is vital. The immunofluorescence assay (IFA) technique is a method that relies on the use of antibodies chemically labeled with fluorescent dyes to visualize molecules under a light microscope, it has become a widely applied tool for the confirmation of equine pathogens due to its rapidity, sensitivity and specificity. Another advantage of IFA is that a large number of samples can be handled simultaneously and stored for certain time. Several IFA methods have been developed for the detection of equine pathogens including direct immunofluorescence and indirect immunofluorescence.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal pathogens for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. Our R&D team who has extensive experience in veterinary diagnostic reagent development, and a series of customized IFA kits can be provided for the detection of equine pathogens according to your specifications. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Equine Pathogens IFA Kits We Can Develop
| Equine herpesvirus direct IFA kit | Equine herpesvirus indirect IFA kit | Equine arteritis virus direct IFA kit |
| Equine arteritis virus indirect IFA kit | Streptococcus equi direct IFA kit | Streptococcus equi indirect IFA kit |
| Streptococcus zooepidemicus direct IFA kit | Streptococcus zooepidemicus indirect IFA kit | Other IFA kits you need |
Workflow of Equine Pathogens IFA Kit Development
| Workflow | Details |
| Sample preparation | Tissue sample or blood sample was taken from the sick horses. |
| Sample fixation | Incubating the sample for 10 minutes at room temperature in a formalin solution or chilled methanol or acetone to crosslink the proteins. |
| Cell permeabilization | To stain intracellular proteins, permeabilize the cell by incubating in a detergent. |
| Blocking | To minimize intra- or extracellular background signals, block the non-specific antigens by incubating the sample in the serum of the host, in which the secondary antibody was made. |
| Antibody incubation | Primary antibody and secondary antibody were incubated in selected solutions successively. |
| Microscopy | Process the microscopic analysis after counterstain and mounting. |
| Assessment of the assay | Evaluate the assay by digital image analysis and statistical analysis. |
Delivery
- A series of IFA kits for equine pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility and repeatability between tests
- High specificity and less nonspecific fluorescent staining
- The multiple targets can be detected in the single sample
- The target with low expression can be detected
- Reasonable price and short turnaround time
References
- Vissani, María Aldana, Armando Mario Damiani, and María Edith Barrandeguy. "Equine Coital Exanthema: new insights on the knowledge and leading perspectives for treatment and prevention." Pathogens 10.8 (2021): 1055.
- Breuil, Marie-France, et al., "Indirect immunofluorescence test using polyclonal antibodies for the detection of Taylorella equigenitalis." Research in Veterinary Science 88.3 (2010): 369-371.
