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Mycoplasma gallisepticum is a bacterium belonging to the class Mollicutes and the family Mycoplasmataceae. It is the pathogen of chronic respiratory disease in chickens and infectious sinusitis in turkeys, chickens, game birds, pigeons, and passerine birds. Mycoplasma gallisepticum attach to host epithelial cells, such as in the respiratory tract, which causes cell damage and inflammatory response.M. gallisepticum can survive in the environment for up to 2 months depending on the conditions. This enables transmission via dust, drinking water and food as well as by direct contact with an infected bird. Some major clinical signs of infection of M. gallisepticumin chickens include those of respiratory distress such as coughing, sneezing, rales, and difficulty breathing.M. gallisepticum can also lead to poor productivity, leg problems, stunting, inappetence, slow growth, reduced hatchability and reduced chick viability, which cause significant economic losses to the poultry industry every year.
Figure 1. Morphology of M. gallisepticum strains (Indikova et al. 2014).
Mycoplasma gallisepticum PCR Kit Development
In order to reduce susceptibility to M. gallisepticum infection and prevent economic losses, vaccination of poultry with M. gallisepticum live vaccines is the most common method. Nevertheless, recurring M. gallisepticum outbreaks necessitated the implementation of rapid and accurate detection of the pathogens. With various biotechnologies being developed, molecular biology techniques are currently used for a rapid detection of M. gallisepticum such as different PCR techniques. Among them, duplex real-time TaqMan PCR is the most widely used because of its high sensitivity and high efficiency.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of Mycoplasma gallisepticum according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Mycoplasma gallisepticum PCR Kit We Can Develop
| M. gallisepticum multiplex PCR Kit | M. gallisepticum real-time PCR Kit |
| M. gallisepticumduplex real-time TaqMan PCR Kit | M. gallisepticum multiplex RT-PCR Kit |
Workflow of Mycoplasma gallisepticum PCR Kit Development
| Workflow | Details |
| Reagents preparation | Prepartaion of the specific reagents for the PCR reaction. |
| Interpretation of real-time PCR | Measurement of reporter dye against the passive reference dye to normalize the fluoresense fluctuations during the cycle. |
| Primers and probes designing | Selection of specific primers and probes for the detection of M. gallisepticum. |
| Sentivity analysis | Evaluation of PCR sensitivity by using 10-fold serial dilution of extracted DNA of M. gallisepticum strain. |
Delivery
- A series of PCR kits for Mycoplasma gallisepticum
- Product quality inspection report
- Other experimental data you need
Our Advantages
- This kit is stable at room temperature
- High sensitivity and specificity
- Compatible with a large number of samples
- Reasonable price and short turnaround time
- Simple and rapid workflow
References
- Bencina, D., Tatjana Tadina, and Dušan Dorrer. "Natural infection of ducks with Mycoplasma synoviae and Mycoplasma gallisepticum and Mycoplasma egg transmission."Avian Pathology17.2 (1988): 441-449.
- Indikova, Ivana, Martin Vronka, and Michael P. Szostak. "First identification of proteins involved in motility of Mycoplasma gallisepticum."Veterinary Research45.1 (2014): 1-14.
