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Clostridium perfringens is a Gram-positive anaerobic rod-shaped bacterium that widely exists in the natural environment and in the intestinal tract of humans and animals. It is an important pathogen of humans and various animals, which can cause necrotizing enteritis, enterotoxemia and other diseases. In recent years, the world's major poultry countries and regions are widespread necrotic enteritis (NE) caused by Clostridium perfringens, especially in Europe and the United States and other developed countries, with the ban on antibiotic growth promoters, the disease is becoming increasingly serious. Clostridium perfringens not only reduces animal production performance and increases chicken mortality, but also causes human infection through poultry meat products, resulting in huge economic losses and human food safety issues. Therefore, the development of a rapid and accurate detection method for Clostridium perfringens is crucial for the control and treatment of NE.
Figure 1. Clostridium perfringens as foodborne pathogen in broiler production: pathophysiology and potential strategies for controlling necrotic enteritis (Mora, et al. 2020).
Clostridium perfringens PCR Kit Development
In recent years, with the development of molecular diagnostic technology, a variety of molecular methods have been used to characterize bacteria, including temperature gradient gel electrophoresis (TGGE), terminal restriction fragment length polymorphism (T-RFLP), denaturing gradient gel electrophoresis Gel electrophoresis (DGGE) and conventional PCR. However, these methods cannot perform quantitative analysis of Clostridium perfringens, which limits their application. In contrast, real-time PCR was used to enumerate Clostridium perfringens in the gastrointestinal tract and feces of animals due to its ability to quantify bacteria. In addition, researchers have developed a variety of PCR methods based on the different detection needs of Clostridium perfringens, including RT-PCR, multiplex PCR, qRT-PCR, IMS-PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of Clostridium perfringens according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Clostridium perfringens PCR Kit We Can Develop
| Clostridium perfringens real-time PCR kit | Clostridium perfringens RT-PCR kit | Clostridium perfringens multiplex PCR kit |
| Clostridium perfringens duplex PCR kit | Clostridium perfringens qRT-PCR kit | Clostridium perfringens IMS-PCR kit |
| Clostridium perfringens nonaplex-PCR kit | Other Clostridium perfringens PCR kits |
Workflow of Clostridium perfringens PCR Kit Development
| Workflow | Details |
| RNA extraction | Purify the virus and extract RNA from Clostridium perfringens-infected cell cultures. |
| Primer design | A set of primers can be designed according to the sequences of specific strains of Clostridium perfringens using professional software. |
| Establishment of PCR conditions | Maintenance of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of RNA from Clostridium perfringens. The sensitivity of the PCR kit was evaluated by determining the limit of detection (LoD). |
Delivery
- A series of PCR kits for Clostridium perfringens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High reproducibility between tests
- Minimal cross-contamination
- Reasonable price and short turnaround time
References
- Mora, Zuamí Villagrán-de la, et al. "Clostridium perfringens as foodborne pathogen in broiler production: pathophysiology and potential strategies for controlling necrotic enteritis." Animals 10.9 (2020): 1718.
- Wu, Shu-Biao, Nicholas Rodgers, and Mingan Choct. "Real-time PCR assay for Clostridium perfringens in broiler chickens in a challenge model of necrotic enteritis." Applied and Environmental Microbiology 77.3 (2011): 1135-1139.
- Si, Weiduo, et al. "Quantification of cell proliferation and alpha-toxin gene expression of Clostridium perfringens in the development of necrotic enteritis in broiler chickens." Applied and Environmental Microbiology 73.21 (2007): 7110-7113.
