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The productivity and profitability of the pig industry around the world are greatly hampered by porcine gastrointestinal infections, which infected farmed pigs from birth until they are ready to be marketed. Pigs typically suffer from viral gastrointestinal infections such as porcine coronaviral enteritis and porcine epidemic diarrhea (PED). The duodenum, jejunum, ileum and colon's epithelial cells are where the PED virus (PEDV) mostly infects and replicates. While porcine gastroenteritis virus infections are currently under control, the recent emergence of virulent PEDV strains results in significant mortality. Piglets all over the world are infected by a variety of viral infections and pig gastrointestinal infections are still widespread in some populations of pigs grown locally.
Figure 1. PEDV dose given to pregnant swine (Vlasova, et al., 2020).
Porcine Gastrointestinal Virus VN Kit Development
Additional assays are needed for effective diagnosis and therapy as acute manifestations can not differentiate between several swine gastrointestinal virus infections. Using fibroblast as targeted cells, the virus neutralization assay inhibits the viral attachment to the cell and reverse transcription and integration. To find group-specific antibodies against gastrointestinal disorders in pigs, the virus neutralization (VN) test has been widely used. The virus neutralization methods have the advantage of being able to accurately evaluate serum samples that have been highly hemolyzed and cytotoxic. In recent years, many antibodies have been found using VN technologies such as plaque reduction neutralization test (PRNT) and serum virus neutralization assay (SVN).
BioVenic is a supplier of biological reagents and kits, we devoted a lot of time to developing diagnostic tools and kits for animal diseases. Our assays are developed and processed with higher standards and the results are delivered on time without compromising quality. We have a team with significant expertise in veterinary diagnostic, and we can provide you with a range of specialized VN kits for the detection of gastrointestinal virus infections of porcine. If you have any needs, please feel free to contact us.
Porcine Gastrointestinal Virus VN Kits We Can Provide
| Porcine gastroenteritis virus PRNT kit | Porcine epidemic diarrhea virus PRNT kit | Porcine gastroenteritis virus SVN kit |
| Porcine epidemic diarrhea virus SVN kit | Other VN kits you need |
Workflow of Porcine Gastrointestinal Virus VN Kit Development
| Workflow | Details |
| Virus titration | Working dilution of the stock virus was determined and the stock virus was aliquoted in multiple tubes and stored at a specific temperature. |
| Dilution | The stock virus was diluted in a virus diluent with trypsin and titration was performed in quadruplicate. |
| Incubation | Incubation was performed at a specific temperature at a given time with a limited supply of carbon dioxide. |
| Cell-fixation | Media was removed from virus titration. |
| Micro-neutralization assay | All serum was treated with receptor destroying enzyme before use in assay and stored at a specific range of temperature for a long term. |
Delivery
- A series of VN kits for porcine gastrointestinal viruses
- Report on product inspection quality
- Other experimental data you need
Our Advantages
- High sensitivity
- The magnitude of functional systematic antibodies can be detected
- High Specificity
- Detect the level of serological cross reactivity
- Reasonable price and short turnaround time
References
- Vlasova, A. N., et al., "Porcine coronaviruses." Emerging and Transboundary Animal Viruses. Springer, Singapore, 2020. 79-110.
- Kitikoon, Pravina, and Amy L. Vincent. "Microneutralization assay for swine influenza virus in swine serum." Animal Influenza Virus. Humana Press, New York, NY, 2014. 325-335.
