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Infectious laryngotracheitis (ILT) is an acute and contagious respiratory disease of chickens. Gallid alpha herpesvirus type 1 (GaHV-1), an enveloped DNA virus is regarded to be the pathogen that cause ILT. Therefore, GaHV-1 is also known as infectious laryngotracheitis virus (ILTV), which is usually spread by infected birds and formites. The main symptoms of infectious laryngotracheitis are severe dyspnea, coughing and rales. Gasping, coughing bloody mucoid exudate, rattling, and extension of the neck could been seen after natural exposure in acute forms. Infectious laryngotracheitis is also reported as a subacute disease characterized by symptoms like tracheitis, nasal and ocular discharge, conjunctivitis, and mild rales. Infectious laryngotracheitis has an overall mortality rate of 5–70% and thus causes immense economic impact in the global poultry industry every year.
Figure 1. Structure of ILTV virion under TEM (Reddy et al., 2016).
Infectious Laryngotracheitis Virus PCR Kit Development
To control infectious laryngotracheitis, ensuring biosecurity and vaccination have been proved to be the main methods. Meanwhile, accurate and rapid detection of infectious laryngotracheitis virus is also of great importance, which is traditionally realized by histopathologic examination of trachea tissue. However, with the rapid development of molecular biology and biotechnology, various PCR techniques have been reported, such as real-time PCR, TaqMan real-time PCR, conventional PCR, qPCR, multiplex real-time RT-PCR and singleplex RT-PCR. These PCR methods are widely used in the rapid detection of infectious laryngotracheitis virus, monitoring and evaluation of treatment efficacy, and detection of infectious agents due to their high sensitivity, good specificity, fast speed and high efficiency.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of infectious laryngotracheitis virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Infectious Laryngotracheitis Virus PCR Kit We Can Develop
| ILTV real-time PCR kit | ILTV conventional PCR kit | ILTV multiplex RT-PCR kit |
| ILTV TaqMan real-time kit | ILTV qRT-PCR kit | ILTV singleplex RT-PCR kit |
Workflow of Infectious Laryngotracheitis Virus PCR Kit Development
| Workflow | Details |
| Design of primers, probes | Professional softwares and databases can be used to design special primers and probes. |
| Preparation of synthetic DNA strands | Preparation of synthetic DNA strands for high quality standardization of PCR assay performance. |
| Processing of ILTV positive specimens | Processing of ILTV-positive samples to validate PCR assay. |
| Thermocycling conditions for PCR | Maintenance of thermocyclic conditions to conduct amplification and detection through PCR. |
| Sensitivity evaluation | Examination of the specificity of primers and probes. |
| Validation of PCR assay | Validation of PCR assay through designated laboratory test and methods. |
Delivery
- A series of PCR kits for the deyection of infectious laryngotracheitis virus (ILTV)
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Each kit is rigorously validated and tested
- Compatible with a large number of samples
- Reasonable price and short turnaround time
References
- Villegas, P. E. D. R. O. "Viral diseases of the respiratory system." Poultry Science 77.8 (1998): 1143-1145.
- Bagust, T. J., R. C. Jones, and J. S. Guy. "Avian infectious laryngotracheitis." Revue Scientifique et Technique (International Office of Epizootics) 19.2 (2000): 483-492.
- Reddy, Avalakuppa Papi, and Vishwanatha Reddy. Novel insights in the pathogenesis of infectious laryngotracheitis and bronchitis viruses in chickens. Diss. Ghent University, 2016.
