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Glaesserella parasuis (G. parasuis, also known as Haemophilus parasuis) is a commensal bacterium of the upper respiratory tract of pigs, belonging to the genus Haemophilus of the family Pasteurelaceae, which is the causative agent of Glässer's disease (GD). GD has significant morbidity and mortality in the global pig herd, and can cause systemic diseases in pigs, such as fibrinous polyserositis, meningitis, arthritis and other diseases. G. parasuis has been reported frequently to cause polyserositis and arthritis in various pig farms, and it has brought huge economic losses to the pig industry worldwide. Therefore, the development of an effective detection method for G. parasuis is crucial for the prevention and treatment of GD and its complications.
Figure 1. Pathology caused by Glaesserella parasuis SV7 strain 174 (Dazzi, et al. 2020).
Glaesserella parasuis PCR Kit Development
G. parasuis is one of the important leading causes of swine mortality worldwide. Studies have shown that different strains of G. parasuis can be isolated from individual pigs or a herd of pigs. G. parasuis was divided into 15 serotypes by indirect hemagglutination test and gel immunodiffusion test, and different serotypes showed different degrees of virulence. Therefore, there is a need to develop accurate and efficient typing methods to identify G. parasuis isolated strains. In recent years, with the continuous development of molecular diagnostic technology, a variety of nucleic acid detection-based methods have been used for the diagnosis of G. parasuis, including ERIC-PCR, multiplex nested PCR, RT-PCR, real-time PCR, Hps-PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of Glaesserella parasuis according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Glaesserella parasuis PCR Kits We Can Develop
| G. parasuis RT-PCR kit | G. parasuis robust PCR kit | G. parasuis real‐time PCR kit |
| G. parasuis multiplex PCR kit | G. parasuis direct PCR kit | G. parasuis ERIC-PCR kit |
| G. parasuisHps-PCR kit | G. parasuis multiplex nested PCR kit | Other G. parasuis PCR kits |
Workflow of Glaesserella parasuis PCR Kit Development
| Workflow | Details |
| Specimen preparation | Specimens can be collected from pigs that were infected by Glaesserella parasuis. |
| Primer and probes design | Available sequences of Glaesserella parasuis can be retrieved from GenBank and specific primer/probe sets were designed. |
| Nucleic acid extraction | Extract RNA from cell cultures, and RNA concentration, purity and integrity were measured. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of RNA from Glaesserella parasuis. The sensitivity of the PCR kit was evaluated by determining the limit of detection (LoD). |
Delivery
- A series of PCR kits for Glaesserella parasuis
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- High sensitivity to detect very small amounts of Glaesserella parasuis in your samples
- Excellent specificity for the detection of Glaesserella parasuis
- Reasonable price and short turnaround time
References
- Dazzi, Cláudia Cerutti, et al. "New pathological lesions developed in pigs by a "non-virulent" strain of Glaesserella parasuis." Frontiers in Veterinary Science 7 (2020): 98.
- Macedo, Nubia, et al. "Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR." Veterinary Research 52.1 (2021): 1-10.
- Moreno, Luisa Zanolli, et al. "ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine." Brazilian Journal of Microbiology 42.4 (2011): 1420-1426.
