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Pig respiratory diseases like enzootic pneumonia (EP) can be caused by a variety of infections. Pig respiratory and reproductive syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), swine influenza virus (SIV), and other viral infections are the cause of porcine respiratory illnesses. These respiratory pathogens can cause symptoms such as high fever, diarrhea, lameness, eye redness, paralysis, etc. The prevalence of swine respiratory diseases costs animal producers a big amount of money each year. Because porcine respiratory diseases are so common, pig farms consistently suffer large financial losses. Vaccination could be a useful approach to keep many of these respiratory diseases to a minimum. However, to treat them properly, the discovery of the corresponding antibodies is crucial.
Figure 1. Structure of PCV2 virus (Kekarainen, et al., 2014).
Porcine Respiratory Virus VN Kit Development
The virus neutralization assay, which is used to effectively treat swine respiratory diseases caused by many pathogens, is used to find antibodies produced in response to infection. The SVN test can be used to assess the degree of serological cross-reactivity between vaccine antisera and swine respiratory viruses, which may be connected with cross-protection in the host. When compared to other techniques, the viral neutralization assay outperforms them in terms of speed, sensitivity, and specificity. Today, a variety of VN techniques are used to detect the antibodies, including the plaque reduction neutralization test (PRNT) and the serum viral neutralization (SVN) assay.
BioVenic is a supplier of biological reagents and kits, we devoted a lot of time to developing diagnostic tools and kits for animal diseases. Our assays are developed and processed with higher standards and the results are delivered on time without compromising quality. We have a team with significant expertise in veterinary diagnostic, and we can provide you with a range of specialized VN kits for the detection of respiratory virus infections of porcine. If you have any needs, please feel free to contact us.
Porcine Respiratory Virus VN Kits We Can Provide
| Porcine circovirus VN kit | Swine influenza virus VN kit | PCV2 virus VN kit |
| PRRSV VN kit | Other VN kits you need |
Workflow of Porcine Respiratory Virus VN Kit Development
| Workflow | Details |
| Preparation of serum | Two-fold serial dilution of the samples was prepared. |
| Preparation of working suspension | On standard quantity, the working suspension was prepared. |
| Incubator operation | At a specific range of temperature, incubation was performed. |
| Mixing of media | The serum-virus mixture was added and plated were again considered for incubation. |
| Record keeping | Neutralizing antibody titer was recorded as the reciprocal of the highest serum dilution. |
Delivery
- Report on product inspection quality
- A series of VN kits for porcine respiratory viruses
- Other experimental data you need
Our Advantages
- High sensitivity
- Reasonable price
- The magnitude of functional systematic antibodies can be detected
- Viruses can be detected at the strain level
- Detect the level of serological cross reactivity
References
- Kekarainen, Tuija, et al., "Genetic variability of porcine circovirus 2 in vaccinating and non-vaccinating commercial farms." Journal of General Virology 95.8 (2014): 1734-1742.
- Allan, Gordon M., and John A. Ellis. "Porcine circoviruses: a review." Journal of Veterinary Diagnostic Investigation 12.1 (2000): 3-14.
