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Astrovirus (AstV) is an unenveloped, single-stranded RNA circular virus that was first identified in 1975 in a stool sample from a child with diarrhea. Since its first discovery, astrovirus has been isolated from a variety of mammalian and avian species. Currently, six different astroviruses have been isolated in birds, are TAstV-1, TAstV-2, ANV, CAstV, DAstV-1 and DAstV-2, based on the species of origin and viral genome characteristics. AstV can infect a variety of poultry and cause outbreaks of intestinal diseases, posing a serious threat to poultry health and causing huge economic losses to the poultry industry. Therefore, it is necessary to develop a rapid and accurate method for detecting AstV.
Figure 1. Genomic architecture of astroviruses (Cortez, et al. 2017).
Avian Astrovirus PCR Kit Development
Avian astroviruses can cause related diseases after infecting most animals, such as poultry enteritis death syndrome, broiler kidney pain, broiler stunting syndrome and fatal hepatitis in ducklings. The detection of astrovirus in most species is limited to electron microscopy (EM) and fluorescent antibody detection. However, the sensitivity of EM is low, and the virus can be detected only in the peak period. Fluorescent antibody test may not be able to identify avian astroviruses with different serotypes. PCR significantly increases the sensitivity of detection compared to EM, fluorescent antibody assays, and enables researchers to develop specific avian astroviruses detection kits. PCR has become a very common tool for diagnosing avian astroviruses infection.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of avian astroviruses according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Avian Astrovirus PCR Kit We Can Develop
| TAstV-1 mPCR kit | TAstV-1 RT-PCR kit | TAstV-1 rRT-PCR kit |
| TAstV-1 qPCR kit | TAstV-1 multiplex RT-PCR kit | TAstV-2 mPCR kit |
| TAstV-2 RT-PCR kit | TAstV-2 rRT-PCR kit | TAstV-2 qPCR kit |
| CAstV PCR kit | CAstV RT-PCR kit | CAstV qPCR kit |
| ANV PCR kit | ANV RT-PCR kit | ANV qPCR kit |
| DAstV-1 PCR kit | DAstV-1 RT-PCR kit | DAstV-1 qPCR kit |
| DAstV-2 PCR kit | DAstV-2 RT-PCR kit | DAstV-2 qPCR kit |
Workflow of RT-PCR Kit Development
| Workflow | Details |
|---|---|
| Viral RNA extraction | Extract the viral RNA from the avian astrovirus positive sample. |
| RNA-polyacrylamide gel electrophoresis (RNA-PAGE) | The extracted RNA of avian astrovirus was subjected to RNA-PAGE. |
| Preparation of complementary DNA | Synthesis cDNA by reverse transcription from avian astrovirus RNA for primer designing. |
| Designing of AstV specific primers | Primer of avian astrovirus can be designed using professional software and database. |
| Optimization of RT-PCR assay | We use a partial plasmid of the specific AstV gene as a template for optimizing and evaluating the sensitivity of the PCR assay. |
| Assessment of PCR efficiency | Independently validate the AstV PCR assay by interlaboratory comparison tests. |
Delivery
- A series of PCR kits for avian astroviruses
- Nucleotide sequence of avian astroviruses
- Primer sequences of avian astroviruses
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Simple and rapid workflow
- Professionals provide you with free consultation
- Reasonable price and short turnaround time
References
- Pérot, Philippe, Marc Lecuit, and Marc Eloit. "Astrovirus diagnostics." Viruses 9.1 (2017): 10.
- Cortez, Valerie, et al. "Astrovirus biology and pathogenesis." Annual Review of Virology 4 (2017): 327-348.
- Ali, Md Zulfekar, et al. "First isolation and molecular characterization of chicken astrovirus and avian nephritis virus in chickens in bangladesh." Frontiers in Veterinary Science 8 (2021).
