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In 1964, William Jarrett and colleagues first observed feline leukemia virus (FeLV) in cats with lymphoma. FeLV is a γ-retrovirus with round particles, 80-120 nm in diameter, and enveloped. The genome of FeLV is a single-stranded positive-strand linear RNA dimer with a length of about 8.3×103nt, including gap, pro-pol and env genes, which mainly encode 9 kinds of proteins. Feline leukemia is one of the most common cancers in cats caused by FeLV, accounting for one-third of all cancers in cats. Although the morbidity rate is only 1-3%, the fatality rate is extremely high. Once infected can die within a few months. Unlike human leukemia, feline leukemia is extremely contagious. Therefore, the development of a rapid detection method for FeLV is very important for the prevention and treatment of feline leukemia.
Figure 1. Formation of FeLV and integration into cells. RT, Reverse transcriptase (Hartmann, Katrin, 2006).
Feline Leukemia Virus PCR Kit Development
Since the first identification of FeLV by electron microscopy (EM), various methods have been reported to detect FeLV, including virus isolation (VI), direct immunofluorescence antibody (IFA), enzyme linked-immunosorbent assay (ELISA) and PCR. VI is the gold standard for the diagnosis of FeLV infection, but this method is time-consuming, labor-intensive, and requires testing in specialized laboratories. In recent years, with the continuous development of molecular detection technology, various PCR methods have been used for the diagnosis of FeLV, such as conventional PCR, real-time PCR, nested PCR, iiPCR, RT-PCR, etc. PCR methods are gradually replacing VI as an alternative method for FeLV diagnosis due to more reliable for detecting FeLV DNA provirus or RNA.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of feline leukemia virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Feline Leukemia Virus PCR Kits We Can Develop
| FeLV conventional PCR kit | FeLV RT-PCR kit | FeLV qPCR kit |
| FeLV real-time PCR kit | FeLV iiPCR kit | FeLV nested PCR kit |
| FeLV qRT-PCR kit | FeLV TaqMan real-time PCR kit | Other FeLV PCR kits |
Workflow of Feline Leukemia Virus PCR Kit Development
| Workflow | Details |
| Specimen preparation | Specimens can be collected from cats that were infected by FeLV. |
| Primer and probes design | Available sequences of FeLV can be retrieved from GenBank and specific primer/probe sets were designed. |
| Nucleic acid extraction | Extract RNA from cell cultures, and RNA concentration, purity and integrity were measured. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of DNA/RNA from FeLV. The sensitivity of PCR kit was assessed by determine the limit of detection (LoD) at the DNA/RNA levels. |
Delivery
- A series of PCR kits for feline leukemia virus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- High sensitivity to detect very small amounts of feline leukemia virus in your samples
- Excellent specificity for the detection of feline leukemia virus
- Reasonable price and short turnaround time
References
- Hartmann, Katrin. "Feline leukemia virus infection." Infectious Diseases of the Dog and Cat 3 (2006): 105-131.
- Wilkes, Rebecca P., et al. "Rapid and sensitive insulated isothermal PCR for point-of-need feline leukaemia virus detection." Journal of Feline Medicine and Surgery 20.4 (2018): 362-369.
