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Canine distemper virus (CDV) is a member of the genus Morbillivirus in the family Paramyxoviridae. Its particles are pleomorphic, most of which are spherical, and there are also deformed and filamentous virus particles. Most CDV particles are between 150 and 330 nm in diameter. The genome of CDV is composed of unsegmented, non-overlapping negative-strand RNA, about 15 690 bp in length. CDV is widely distributed worldwide, and domestic dogs are the main host for CDV infection. In addition, CDV can also infect wild animals, tigers, lions, leopards and seals. Canine distemper (CD) is a highly contagious infectious disease caused by CDV infection, with high infectivity and morbidity, and various clinical symptoms. It is easy to form secondary and mixed infections in the later stage of infection. The disease has caused great losses to the fur economy animal breeding industry and rare wild animals around the world.
Figure 1. Anatomic sites targeted by CDV infection (Sykes, et al. 2013).
Canine Distemper Virus PCR Kit Development
CDV diagnostics help researchers solve many of the challenges of CDV pathogenesis, transmission patterns and wildlife conservation. Various methods have been developed to diagnose CDV, including virus isolation, electron microscopy, histology, immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). In recent years, with the advancement of molecular detection technology, a large number of PCR detection methods for CDV diagnosis have been described, such as real-time RT-PCR, nested RT-PCR, insulated isothermal PCR (iiPCR) and reverse transcription polymerase chain reaction (RT-PCR). These methods have different sensitivity and specificity and need to be selected as appropriate and effective depending on the samples tested to accurately identify CDV infection for early diagnosis and epidemiological surveillance of CD.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of canine distemper virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Canine Distemper Virus PCR Kit We Can Develop
| CDV conventional PCR kit | CDV RT-PCR kit | CDV real-time PCR kit |
| CDV nested PCR kit | CDV iiPCR kit | CDV one-step duplex PCR kit |
| CDV RT-qPCR kit | CDV multiplex RT-PCR Kit | Other CDV PCR Kit |
Workflow of Canine Distemper Virus PCR Kit Development
| Workflow | Details |
| Samples and cell cultures | Samples were obtained from naturally infected dog, CDV strains were grown in dog cells. |
| Primer and probes design | Design primers or probes to detect CDV, and use professional software to select and evaluate primers and probes. |
| Nucleic acid extraction | Extract RNA from nasopharyngeal/conjunctival swabs, urine and tissues of infected dogs. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | Other pathogens such as canine parvovirus are tested to assess the specificity of PCR kits. Determine the minimum amount of CDV detected in the sample to assess the sensitivity of the PCR kit. |
Delivery
- A series of PCR kits for canine distemper virus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High purity Taq DNA Polymerase
- Highly specific detection profile
- High reproducibility between tests
- Reasonable price and short turnaround time
- Professional R&D personnel and technology platform
References
- Sykes, Jane E. "Canine distemper virus infection." Canine and Feline Infectious Diseases. Elsevier, St. Louis, Missouri (2013): 152-165.
- Tomaszewicz Brown, Ania, et al. "Development and validation of a portable, point-of-care canine distemper virus qPCR test." PLoS One 15.4 (2020): e0232044.
- Wilkes, Rebecca P., et al. "Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction." BMC Veterinary Research 10.1 (2014): 1-8.
