Enzyme-linked immunosorbent assay (ELISA) is an immunological method that uses the catalytic amplification effect of enzymes based on the specific reaction of antigen and antibody, and can be widely used for the detection of antigen and antibody, with the advantages of high specificity, high sensitivity, easy operation, objective and accurate results, and high safety. In the assay, antibodies are first coated on a 96-well plate made of polystyrene and then used to recognize the antigen (protein biomarkers of disease, viruses, bacteria, etc.) to be tested and adsorb the antigen from the sample to be measured onto the surface of the 96-well plate. Then, the recognition signal is output by direct or indirect means using antibodies with horseradish peroxidase (HRP), fluorescent or radiolabeled. Finally, the concentration of the target antigen in the sample to be tested is calculated using information such as signal intensity and the concentration gradient of the standard sample. According to the different ways of immune recognition and signal output, ELISA can be divided into double antibody sandwich ELISA, direct ELISA and indirect ELISA, etc. Among them, the double antibody sandwich ELISA is the most common in commercial applications.
Figure 1. The process of indirect ELISA.
Veterinary ELISA Tests
ELISA method is considered the gold standard of immunoassays. It can quantitatively or qualitatively measure the level of antibodies in animal samples. It is widely used for the veterinary research and discover the potential mechanisms of animal diseases or explore the pathogenesis of bacteria and virus among animals.
BioVenic is a supplier of biological reagents and kits for veterinary research use. We have a professional antibody and antigen development technology platform and research team who have extensive experience in veterinary immunoassay development. If you have any veterinary research requirements, please feel free to contact us. We are here to support you for your veterinary research and animal-related immunoassay development.
