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Bovine respiratory disease (BRDC) is a disease caused by multiple factors, in which a series of host and environmental factors make animals vulnerable to various viral and bacterial pathogens. The main viral pathogens include bovine herpesvirus type 1 (BHV-1), bovine parainfluenza virus type 3 (BPIV-3), bovine respiratory syncytial virus (BRSV) and bovine viral diarrhea virus (BVDV). The most common bacterial pathogens Mannheimia haemolytica, Pasteurella multocida and Histophilus somni. These bacteria commonly exist in cattle as symbiotic nasopharyngeal flora, but they can aggravate the disease through secondary infection of the lower respiratory tract. Many other organisms, Mycoplasma spp., may also be associated with BRDC. BRDC has become a significant cause of mortality and production losses in cattle, especially in the case of feedlots.
Figure 1. Factors affecting the development of the bovine respiratory microbiota (Zeineldin, et al., 2019).
Bovine Respiratory Pathogens AGID Kit Development
Since the symptoms of BRDC caused by different pathogens have many similarities, the identification of bovine respiratory pathogens is very important. Agar gel immunodiffusion is a kind of useful and widely applied serological method for the indentification of bovine respiratory pathogens, which includes a variety of techniques that can be used to detect host exposure to a pathogen or to serotype field isolates. Several AGID techniques have been developed for the detection of bovine respiratory pathogens, including radial immunodiffusion assay, single immunodiffusion assay, double immunodiffusion assay, etc.
BioVenic is a leading company in the field of biotechnology services, able to provide the customers with rapid access to animal diseases diagnostic services. We have a professional and experienced R&D team, who is working hard at developing and optimizing agar gel immunodiffusion kits. A variety of customized agar gel immunodiffusion kits for the identification of bovine respiratory pathogens can be provided according to your specifications. If you have any needs, please do not hesitate to contact us. We will provide you with high-quality products and one-stop services.
Bovine Respiratory Pathogens AGID Kits We Can Develop
| BHV-1 radial immunodiffusion enzyme assay kit | BHV-1 single immunodiffusion assay kit | BHV-1 double immunodiffusion assay kit |
| BPIV-3 radial immunodiffusion enzyme assay kit | BPIV-3 single immunodiffusion assay kit | BPIV-3 double immunodiffusion assay kit |
| Mannheimia haemolytica radial immunodiffusion enzyme assay kit | Mannheimia haemolytica single immunodiffusion assay kit | Mannheimia haemolytica double immunodiffusion assay kit |
| Pasteurella multocida radial immunodiffusion enzyme assay kit | Pasteurella multocida single immunodiffusion assay kit | Other AGID kits you need |
Workflow of Bovine Respiratory Pathogens AGID Kit Development
| Workflow | Details |
| Sample preparation | Serum is separated by centrifugation from blood sample taken from sick cattle, and fresh serum can be directly used for AGID detection |
| Preparation of agarose gel | Agarose gel was prepared in a specific buffered saline solution at a specific temperature for a given period. |
| Preparation of agar plates | Dispense the melted agar onto a flat surface and prepare the agar plate at a specific temperature |
| Agar gel immunodiffusion for antibody detection | For each batch of specimens, a strong positive, weak positive, and negative reference serum will be included to aid in interpreting results |
| Interpretation of test results for antibody detection | Interpret the result via different analysis methods and evaluate the efficiency of the assay. |
Delivery
- A series of AGID kits for bovine respiratory pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- Excellent diagnostic relative sensitivity and specificity
- Reasonable price and short turnaround time
- Combine AGID with other detection methods to avoid false positives
References
- Hick, P. M., et al. "Coronavirus infection in intensively managed cattle with respiratory disease." Australian Veterinary Journal 90.10 (2012): 381-386.
- Zeineldin, Mohamed, James Lowe, and Brian Aldridge. "Contribution of the mucosal microbiota to bovine respiratory health." Trends in Microbiology 27.9 (2019): 753-770.
