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Bovine pathogens are the leading cause of death and illness, contributing to millions of dollars in losses through treatment costs, reduced meat yields, and mortalities. The term bovine pathogens can cover a range of pathogens, from fatal respiratory disease-causing pathogens to gastrointestinal disease-causing pathogens. Multiple viral and bacterial agents are implicated in bovine diseases, including bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine herpesvirus 1 (BoHV-1), parainfluenza 3 virus (PI3V), Mannheimia haemolytica, Mycoplasma bovis, Pasteurella multocida, and Histophilus somni. These bovine pathogens are also threatening the health of dairy cows and causing a variety of diseases in cows, posing a danger to the worldwide dairy sector.
Figure 1. Helper T cell responses to infections in cattle (Kandel, et al.,2021).
Bovine Pathogens IFA Kit Development
The use of pathogen isolation and display of antigen techniques to diagnose highly bovine pathogens quickly and accurately is a critical component of effective disease control. Immunofluorescence is a biological technique for analyzing molecules using antibodies that have been chemically tagged with fluorescent dyes. Immunofluorescence assay (IFA) is responsible for the study of distribution of specific protein antigens in cells that combines immunological approaches and fluorescent labelling technology. IFA can be used to examine the distribution of proteins, glycans, and tiny biological and non-biological antigens in tissue slices, cultured cell lines, or individual cells. Direct immunofluorescence and indirect immunofluorescence are two IFA approaches that have been developed for the detection of bovine infections.
For many years, BioVenic has been focused on creating diagnostic reagents and kits for animal diseases. We have a skilled team with experience in veterinary diagnostics and kit development. Our R&D team can develop a series of customized immunofluorescence kits for the identification of bovine pathogens based on your requirements. Please do not hesitate to contact us if you have any requirements. We will deliver high-quality goods and services to you.
Bovine Pathogens IFA Kits We Can Develop
| Mannheimia haemolytica direct IFA kit | Mannheimia haemolytica indirect IFA kit | Pasteurella multocida direct IFA kit |
| Pasteurella multocida indirect IFA kit | Mycoplasma bovis direct IFA kit | Mycoplasma bovis indirect IFA kit |
| BVDV direct IFA kit | BVDV indirect IFA kit | Other IFA kits you need |
Workflow of Bovine Pathogens IFA Kit Development
| Workflow | Details |
| Sample preparation | Tissue sample or blood sample was taken from the cattle. |
| Sample fixation | Incubating the sample for 10 minutes at room temperature in a formalin solution or chilled methanol or acetone to crosslink the proteins. |
| Cell permeabilization | To stain intracellular proteins, permeabilize the cell by incubating in a detergent. |
| Blocking | To minimize intra- or extracellular background signals, block the non-specific antigens by incubating the sample in the serum of the host, in which the secondary antibody was made. |
| Antibody incubation | Primary antibody and secondary antibody were incubated in selected solutions successively. |
| Microscopy | Process the microscopic analysis after counterstain and mounting. |
| Assessment of the assay | Evaluate the assay by digital image analysis and statistical analysis. |
Delivery
- A series of IFA kits for bovine pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- High specificity and less nonspecific fluorescent staining
- The multiple targets can be detected in the single sample
- Reasonable price and short turnaround time
References
- Wood, Caitlin, et al., "Validation of an indirect immunofluorescence assay (IFA) for the detection of IgG antibodies against Coxiella burnetii in bovine serum." Preventive veterinary medicine 169 (2019): 104698.
- Von dem Borne, AEG Kr, et al., "A simple immunofluorescence test for the detection of platelet antibodies." British Journal of Haematology 39.2 (1978): 195-207.
- Kandel, Anmol, et al., "CD4+ T Cell Responses to Pathogens in Cattle." Bovine Science-Challenges and Advances. IntechOpen, 2021.
