Services
Pseudorabies virus (PRV), also known as porcine herpesvirus type I, belongs to the alpha-herpesvirus subfamily of the Herpesviridae family and has oval or round viral particles and a linear double-stranded DNA molecule with a genome of approximately 150 kb. PRV is the causative agent of pseudorabies (PR) and can cause infection in a variety of domestic and wild animals. Pigs are the primary host of infection and source of infection for this virus. PR is one of the most important diseases threatening the pig industry at present. After infection with PRV, sows show reproductive disorders, newborn piglets show neurological symptoms and up to 100% mortality, and fattening pigs show respiratory disease. PRV has caused serious economic losses to the pig industry worldwide and is classified as a category B infectious disease by the World Organization for Animal Health (OIE).
Figure 1. Structure of a pseudorabies virus (Nauwynck, et al. 2007).
Pseudorabies Virus ELISA Kit Development
Pseudorabies is a highly infectious disease, and its worldwide control and eradication is necessary to reduce economic losses. Currently, several serological method assays have been developed for monitoring PR, including serum neutralization test (SNT), enzyme-linked immunosorbent assay (ELISA) and direct immunofluorescence assay (DFM). Among them, ELISA is widely used for PRV detection because of its higher sensitivity, higher specificity and ease of standardization.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare monoclonal antibodies specific for individual antigens of pseudorabies virus or antibodies against whole virus multi-antigens, so we can provide you with a range of customized ELISA kits for pseudorabies virus detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| PRV gB-ELISA kit | PRV gC-ELISA kit |
| PRV gD-ELISA kit | PRV gE-ELISA kit |
| PRV whole-virus ELISA | PRV gG-ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
|---|---|
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | DNA extraction, cloning and sequencing of genes encoding the proteins B, C, D and E of PRV, and then constructing vectors to express antigens. |
| Antibody preparation | Preparation of antibodies specific for proteins B, C, D and E of PRV. |
| ELISA kit development | Antibody label, antibody paired screening, method development and optimization, kit production. |
| ELISA kit delivery | We will provide you with customized ELISA kits and complete product quality inspection report. |
Delivery
- A series of ELISA kits for detection pseudorabies virus
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Each kit is rigorously validated and tested
- Professional antibody platform to support ELISA kit development
- Reasonable price and short turnaround time
References
- Nauwynck, Hans, et al. "Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract." Veterinary Research 38.2 (2007): 229-241.
- Wu, C‐Y., et al. "Enhancing expression of the pseudorabies virus glycoprotein E in yeast and its application in an indirect sandwich ELISA." Journal of Applied Microbiology 123.3 (2017): 594-601.
- Cheng, Ting-Yu, et al. "Detection of pseudorabies virus antibody in swine oral fluid using a serum whole-virus indirect ELISA." Journal of Veterinary Diagnostic Investigation 32.4 (2020): 535-541.
