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Chlamydia psittaci (C. psittaci) is a small gram-negative bacterium with a diameter of 0.3 μm to 0.5 μm. Psittacosis, also known as ornithosis, is an infectious diseases caused by the infection of C. psittaci in birds, humans and some mammals. The disease once broke out in Europe, and the pathogen was first isolated from sick birds in 1893. In 1894, the sick parrot was confirmed as the source of infection, so the disease was named psittacosis. C. psittaci is transmitted through direct contact with infected bird feces, nasal secretions and aerosols, which can remain infectious in the environment for several months. The widespread spread of C. psittaci has serious implications for poultry farming economies and human health. Therefore, it is necessary to find an effective method to detect C. psittaci.
Figure 1. Pulmonary lesions (A–D) and model of local immune response to Chlamydiae (E). Pulmonary lesions 4 days after experimental inoculation of a calf (A and B) and a mouse (C and D) with C. psittaci (Knittler, et al. 2014).
Chlamydia psittaci PCR Kit Development
Inferring possible avian sources through accurate diagnosis of C. psittaci can help monitor and manage the spread of psittacosis. C. psittaci is often is often detected by culture and serological testing. However, culture is a time-consuming and labor-intensive method, and C. psittaci is infectious and needs to be performed in a laboratory with a higher level of security. In addition, serological tests are less specific, may cross-infect with other mycoplasmas, and require testing sera collected several weeks apart, so the diagnosis results cannot be obtained in time. In recent years, molecular biology techniques have been rapidly developed and used for the diagnosis of C. psittaci. Currently, several PCR methods have been developed for the detection of C. psittaci, including real-time PCR, RT-PCR, ddPCR, multiplex PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of C. psittaci according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Chlamydia psittaci PCR Kits We Can Develop
| C. psittaci real-time PCR kit | C. psittaci RT- PCR kit | C. psittaci PCR-QC kit |
| C. psittaci multiplex PCR kit | C. psittaci ddPCR kit | C. psittaciTETR-PCR kit |
| C. psittaci qRT-PCR kit | Other C. psittaci PCR kits |
Workflow of Chlamydia psittaci PCR Kit Development
| Workflow | Details |
| Specimen preparation | Specimens can be collected from animals that were infected by C. psittaci. |
| Primer and probes design | Available sequences of C. psittaci can be retrieved from GenBank and specific primer/probe sets were designed. |
| Nucleic acid extraction | Extract RNA from cell cultures, and RNA concentration, purity and integrity were measured. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of RNA from C. psittaci. The sensitivity of the PCR kit was evaluated by determining the limit of detection (LoD). |
Delivery
- A series of PCR kits for C. psittaci
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- High sensitivity to detect very small amounts of C. psittaci in your samples
- Excellent specificity for the detection of C. psittaci
- Reasonable price and short turnaround time
References
- Knittler, Michael R., et al. "Chlamydia psittaci: New insights into genomic diversity, clinical pathology, host–pathogen interaction and anti-bacterial immunity." International Journal of Medical Microbiology 304.7 (2014): 877-893.
- Ménard, Armelle, et al. "Development of a real-time PCR for the detection of Chlamydia psittaci." Journal of Medical Microbiology 55.4 (2006): 471-474.
- Hisada, Kyoko, et al. "Development and evaluation of a novel quenching probe PCR (GENECUBE) assay for rapidly detecting and distinguishing between Chlamydia pneumoniae and Chlamydia psittaci." Journal of Microbiological Methods 184 (2021): 106212.
