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Avian respiratory diseases can be caused by viruses, bacteria and mycoplasma, and are a common type of disease in poultry farming. Avian respiratory pathogens are diverse and include avian pneumovirus (APV), avian influenza virus (AIV), avian paramyxovirus (APMV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), Avibacterium paragallinarum (A. paragallinarum), Ornithobacterium rhinotracheale (ORT), Mycoplasma synovialis, Mycoplasma gallisepticum (MG), etc. These respiratory pathogens can cause avian respiratory diseases independently or interactively, and this interaction may be synergistic or antagonistic, which determines the severity of the disease complex. Avian respiratory disease has a complex etiology and is often caused by multiple pathogens.
Figure 1. Structure of ILT virus (Gowthaman, et al., 2020)
Avian Respiratory Pathogens AGID Kit Development
Poor management of avian respiratory diseases can lead to high mortality and cause significant economic losses to public health and the poultry farming industry. Therefore, the development of simple and rapid detection methods is essential for the prevention and control of poultry respiratory diseases. In recent years, with the advancement of medical science and biotechnology, various methods have been developed to detect different avian respiratory pathogens. Agar gel immunodiffusion (AGID) is a simple and economical serological test commonly used to screen poultry flocks for infection with respiratory pathogens. Each test has advantages and disadvantages so that researchers need to consider these factors before selecting the best test.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal pathogens for many years. Our goal is to be a great company that improves the health and well-being of pets, humans and livestock. We have a professional team with extensive experience in veterinary diagnostics and reagent development, and our R&D team is working hard to develop and improve AGID kits. We can provide you with a series of customized AGID kits for the detection of avian respiratory pathogens according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Avian Respiratory Pathogens AGID Kits We Can Provide
| Avian pneumovirus AGID kit | Avian influenza virus AGID kit |
| Avian paramyxovirus AGID kit | Infectious bronchitis virus AGID kit |
| Infectious laryngotracheitis virus AGID kit | Avibacterium paragallinarum AGID kit |
| Ornithobacterium rhinotracheale AGID kit | Mycoplasma synovialis AGID kit |
| Mycoplasma gallisepticum AGID kit | Other AGID kit |
Workflow of Avian Respiratory Pathogens AGID Kit Development
| Workflow | Details |
| Cell culture | Avian cells chronically infected with avian respiratory pathogens were cultured in medium containing 10% fetal bovine serum, and cell culture supernatants were collected and concentrated to obtain AGID avian respiratory pathogen antigens. |
| Cloning and expression of recombinant genes | Extract genomic DNA, clone recombinant genes and detect recombinant gene expression. |
| Preparation of recombinant antigens | Preparation of recombinant antigen and determination of the concentration of recombinant antigen. |
| AGID testing | The designated reference serum was tested with AGID to assess the reproducibility of the AGID assay. |
Delivery
A series of AGID kits for avian respiratory pathogens
Product quality inspection report
Other experimental data you need
Our advantages
- Negative sera neither form a line with the reference positive control line
- With excellent repeatability and reproducibility
- Inexpensive and high specificity
- Simple and rapid workflow
References
- Jenson, Terra A. "Agar gel immunodiffusion assay to detect antibodies to type A influenza virus." Animal Influenza Virus. Humana, New York, NY, 2020. 165-175.
- Gowthaman, Vasudevan, et al. "Infectious laryngotracheitis: Etiology, epidemiology, pathobiology, and advances in diagnosis and control–a comprehensive review." Veterinary Quarterly 40.1 (2020): 140-161.
