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In recent years, the breeding of cattle, sheep, goats and other ruminant is almost always carried out by artificial insemination or fertilized egg transplantation. However, the conception rate brought by artificial insemination and fertilized egg transplantation is not high. The low conception rate causes an increase in the number of artificial insemination or fertilized egg transplantation, the period of empty fetuses without pregnancy and the prolongation of the interval between deliveries, causing a serious economic burden to the livestock industry. Early pregnancy diagnosis in ruminants is an important measure to shorten the calving interval to ensure high reproductive efficiency and reduce economic losses. Therefore, it is necessary to find simple, rapid and accurate methods for early pregnancy detection in ruminants.
Figure 1. Representative patterns of PAG immunolocalization in bovine placentome sections. Some variation in the localization of PAGs is apparent in bovids(Wallace, et al.2015).
Ruminant Pregnancy ELISA Kit Development
Pregnancy judgment of ruminants is mainly carried out by rectal detection method and ultrasonic diagnosis method. Although the reliability of the judgment results of either method is high, it must be carried out more than one month after fertilization. This prolongs the non-pregnant void and calving interval in ruminants, resulting in lower reproductive rates. ELISA is a simple and rapid method for early pregnancy diagnosis in ruminants, which can be used to detect pregnancy markers in milk and serum.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare antibodies specific for pregnancy markers in milk and serum of ruminant, and we can provide you with a range of customized ELISA kits for ruminant pregnancy detection according to your needs. If you have any needs, please feel free to contact us, we will provide you with high-quality products.
The ELISA Kits We Can Develop
| Progesterone ELISA kit | Estrone sulphate ELISA kit | Early conception factor ELISA kit |
| Interferon-𝛕 ELISA kit | PAG ELISA kit | Other ruminant pregnancyELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Preparation of proteins for immunizations, purification recombinant protein. |
| Antibody preparation | Preparation of antibodies specific for pregnancy markers in early pregnancy of ruminant. |
| ELISA kit development | Optimizing the final reaction conditions, evaluation of sensitivity, specificity and concordance of ELISA kit. |
| ELISA kit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection ruminant pregnancy
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Ready-to-use liquid reagents make assays easy to implement and run
- High sensitivity and specificity
- High repeatability between tests
- Professional antibody platform to support ELISA kit development
- Reasonable price and short turnaround time
References
- Krebs, Tony, et al. "Establishment of a Sandwich-ELISA for simultaneous quantification of bovine pregnancy-associated glycoprotein in serum and milk." PloS One 16.5 (2021): e0251414.
- Balhara, Ashok K., et al. "Early pregnancy diagnosis in bovines: current status and future directions." The Scientific World Journal 2013 (2013).
- Wallace, Rhianna M., et al. "Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy." Reproduction 149.3 (2015): R115-26.
