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The gastrointestinal (GI) system is more prone to disease than any other part of the sheep or goat. Many species of nematodes and cestodes cause parasitic gastritis and enteritis in sheep. The most important of them are Haemonchus contortus, Teladorsagia circumcinta, Trichostrongylus axei, intestinal species of Trichostrongylus, Nematodirus spp. Coccidiosis of sheep and GI helminthiasis are important causes of diarrhea in older nursing and weaned sheep. The serotypes of enteropathogenic Escherichia coli may cause secretory diarrhea in sheep. Similarly, rotavirus, coronavirus and cryptosporidia also cause outbreaks of diarrhea in lambs. Enterotoxemia is a frequent severe disease of sheep of all ages, which is caused by two strains of Clostridium perfringens. Sheep gastrointestinal disease alone has become one of the most significant causes of production and animal losses.
Figure 1. Pathogenesis of C. perfringens in sheep(Pawaiya et al., 2020).
Sheep Gastrointestinal Pathogens AGID Kit Development
Due to the clinical signs of sheep gastrointestinal diseases can be vague sometimes, identification of the pathogens is crucial for differentiating gastrointestinal diseases, developing a prognosis and plan for treatment, and monitoring the treatment. Agar gel immunodiffusion (AGID) is a diagnostic technique for the detection or measurement of antibodies and antigens by their precipitation which involves diffusion through a substance such as agar or gel agarose. AGID is a stable method, which can detect cross-reactions between antigens in a mixture. AGID has been used as an efficient tool in laboratories for the diagnosis of various pathogens. For the detection of sheep gastrointestinal pathogens, single immunodiffusion, double immunodiffusion, radial immunodiffusion and Ouchterlony double immunodiffusion are commonly used assays.
BioVenic is a supplier of biological reagents and kits, we are committed to fulfilling all your demands for agar gel immunodiffusion (AGID) kits. Our R&D team is working hard to provide high-quality reagents and comprehensive solutions to support your innovative discoveries and diagnosis of animal pathogenic infections. If you have any needs in the development of sheep gastrointestinal pathogens AGID kit, please feel free to contact us. We will provide you with premium products and one-stop services.
Sheep Gastrointestinal Pathogens AGID Kits We Can Develop
| Haemonchus contortus radial immunodiffusion assay kit | Haemonchus contortus single immunodiffusion | Teladorsagia circumOuchterlony double immunodiffusion assay kit |
| Teladorsagia circumsingle immunodiffusion assay kit | Clostridium perfringens double immunodiffusion assay kit | Clostridium perfringens single immunodiffusion |
| Rotavirus single immunodiffusion assay kit | Rotavirus double immunodiffusion assay kit | Other AGID kits you need |
Workflow of Sheep Gastrointestinal Pathogens AGID Kit Development
| Workflow | Details |
| Collection of samples | Blood was collected from sick sheeps and left to clot at a specific temperature. |
| Agar gel immunodiffusion | Agar gel was prepared and diluted with distilled water at specific pH and a given range of temperatures. |
| AGID reading and Interpretation | AGID analysis was read and interpreted between the lines observed in the diagnostic assay. |
| Data analysis | Data was analyzed in the form of tables. |
| Result analysis | The result was analyzed after 48 hours of the initiation of the reaction assay. |
Delivery
- A set of AGID kits for sheep gastrointestinal infections
- Product quality control
- List of other experimental data you need
Our Advantages
- Short turnaround time and resonable price
- Detecting a small number of sheep gastrointestinal infections in a high-sensitivity manner
- Detecting therapeutic pathogens with a better level of specificity
- Combine AGID with other detection methods to avoid false positives
References
- Pawaiya, Rajveer Singh, et al. "The Challenges of Diagnosis and Control of Enterotoxaemia Caused by Clostridium perfringens in Small Ruminants." Advances in Microbiology 10.5 (2020): 238-273.
- Morgan, E. R., and J. Van Dijk. "Climate and the epidemiology of gastrointestinal nematode infections of sheep in Europe." Veterinary Parasitology 189.1 (2012): 8-14.
- Roeber, Florian, Aaron R. Jex, and Robin B. Gasser. "Impact of gastrointestinal parasitic nematodes of sheep, and the role of advanced molecular tools for exploring epidemiology and drug resistance-an Australian perspective." Parasites & Vectors 6.1 (2013): 1-13.
