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BTV belongs to the genus Orbivirus of the family Reoviridae, its genome consists of ten double-stranded RNA segments encoding seven structural (VP1-7) and four nonstructural (NS1-4) proteins. It is classified into 27 serotypes based on the genetic and antigenic characteristics of the neutralizing protein VP2. Bluetongue (BT) is a non-contact insect-borne disease caused by BTV that is susceptible to a wide range of ruminants and can infect a variety of domestic and wild ruminants, with sheep being the most susceptible. The clinical signs of BTV infection vary greatly depending on the serotype of the virus, the type of animal infected and whether the disease is first prevalent. BT has brought great harm to animal husbandry and caused huge economic losses all over the world.
Figure 1. Representative scheme of BTV structural proteins and dsRNA segments (Schwartz-Cornil, et al. 2008).
Bluetongue Virus PCR Kit Development
BTV was originally identified and distinguished from other circoviruses by serological methods, including virus neutralization tests (VNT), agar gel immunodiffusion (AGID) tests, serum neutralization tests (SNT), and enzyme-linked immunosorbent assays (ELISA). However, serological assays are expensive, time-consuming and have poor sensitivity. With the continuous development of molecular biology, researchers have developed several PCR methods for the detection of BTV, including RT-PCR, real-time PCR and multiplex PCR. Compared with serological methods, PCR has significant advantages of speed, sensitivity and specificity obvious advantage.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of bluetongue virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Bluetongue Virus PCR Kit We Can Develop
| BTV-1 RT-PCR kit | BTV-1 real-time PCR kit | BTV-1 multiplex RT-PCR kit |
| BTV-2 RT-PCR kit | BTV-2 real-time PCR kit | BTV-2 multiplex RT-PCR kit |
| BTV-4 RT-PCR kit | BTV-4 real-time PCR kit | BTV-4 multiplex RT-PCR kit |
| BTV-6 RT-PCR kit | BTV-6 real-time PCR kit | BTV-6 multiplex RT-PCR kit |
| BTV-8 RT-PCR kit | BTV-8 real-time PCR kit | BTV-8 multiplex RT-PCR kit |
| Othet BTV RT-PCR kit | Othet BTV real-time PCR kit | Othet BTV multiplex RT-PCR kit |
Workflow of Bluetongue Virus PCR Kit Development
| Workflow | Details |
| RNA extraction | Purify the virus and extract RNA from bluetongue virus infected cell cultures. |
| Primer and probes design | Unique regions in each BTV serotype Seg-2 are identified as targets for primers and probes. Primers and probes specific to each BTV serotype are then designed. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | Evaluation of reproducibility of PCR by comparing the bluetongue virus positive samples from different sources. |
| Assessment of sensitivity and specificity | Evaluation of the diagnostic specificity, sensitivity and efficiency of PCR assays. |
Delivery
- A series of PCR kits for bluetongue virus
- Primer sequences of bluetongue virus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High reproducibility between tests
- High priming efficiency
- Broad dynamic detection range
References
- Maan, Sushila, et al. "Development and evaluation of real time RT-PCR assays for detection and typing of bluetongue virus." PLoS One 11.9 (2016): e0163014.
- Schwartz-Cornil, Isabelle, et al. "Bluetongue virus: virology, pathogenesis and immunity." Veterinary Research 39.5 (2008): 1.
