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In recent years, several clinical cases and epidemiological studies of feline diseases have been reported worldwide. The diseases associated with cats include rabies, capnocytophagosis, pasteurellosis, cat scratch disease, ringworm, sporotrichosis, tularemia, plague, Q fever and external parasites, campylobacterosis, salmonellosis, infections with pathogenic E. coli, cryptosporidiosis, giardiasis, toxoplasmosis, and MRSA. Feline diseases can be caused by various pathogens. Capnocytophaga canimorsus and Pasteurella multocida are bacteria that are commonly responsible for rabies. Cat scratch disease caused by Bartonella hensalae is a bacterial infection that is transmitted by bites, scratches, and flea bites. Viral pathogens such as feline coronavirus (FCoV), feline leukemia virus (FeLV) and feline panleukopenia virus (FPV) may lead to multiple cat infections. Typical signs include sneezing, nasal discharge, ocular discharge, conjunctivitis, ulceration of the tongue, lethargy, inappetence and fever. To develop procedures and therapeutic strategies for the control of specific pathogens. it's important to identify the feline pathogens.
Figure 1. Vaccination against the feline immunodeficiency virus (Lecollinet, et al., 2008).
Feline Pathogens IFA Kit Development
With the emergence of novel feline pathogens, it is very important to establish new methods or improve the already existing techniques in the diagnostic field. Immunofluorescence is a powerful technique that utilizes fluorescent-labeled antibodies to detect specific target antigens. It is used widely in both scientific research and clinical laboratories. Immunofluorescence assay (IFA) is a microscope-based technique used clinically to diagnose certain cutaneous diseases by detection of autoantibody-antigen complexes. There are two main types of commonly used IFA for the detection of feline pathogens, including direct IFA and indirect IFA. Direct IFA (primary IFA) involves a single antibody and fluorophore directly conjugated to this antibody, while indirect IFA (secondary IFA) involves two antibodies (primary and secondary) and fluorophore conjugated to the secondary antibody.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal pathogens for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. We can provide you with a series of customized immunofluorescence kits for the detection the identification of feline infections based on your requirements. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Feline Pathogens IFA Kits We Can Develop
| E. coli direct IFA kit | E. coli indirect IFA kit | Bartonella hensalae direct IFA kit |
| Bartonella hensalae in direct IFA kit | Pasteurella multocida direct IFA kit | Pasteurella multocida indirect IFA kit |
| FCoV direct IFA kit | FCoV indirect IFA kit | Other IFA kits you need |
Workflow of Feline Pathogens IFA Kit Development
| Workflow | Details |
| Preparation of sample | A tissue sample or blood sample was taken from the sick cats. |
| Sample fixation | The sample was incubated at room temperature in a formalin solution or acetone to crosslink the proteins. |
| Cell permeabilization | To stain intracellular proteins, permeabilize the cell by incubating in a detergent. |
| Blocking | To minimize intra- or extracellular background signals, block the non-specific antigens by incubating the sample in the serum of the host, in which the secondary antibody was made. |
| Antibody incubation | Primary antibody and secondary antibody were incubated in selected solutions successively. |
| Microscopy | Process the microscopic analysis after counterstain and mounting. |
| Assessment of the assay | Evaluate the assay by digital image analysis and statistical analysis. |
Delivery
- A series of IFA kits for feline pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- High specificity and less nonspecific fluorescent staining
- The multiple targets can be detected in the single sample
- Reasonable price and short turnaround time
References
- Lecollinet, Sylvie, and Jennifer Richardson. "Vaccination against the feline immunodeficiency virus: the road not taken." Comparative Immunology, Microbiology and Infectious Diseases 31.2-3 (2008): 167-190.
- Odell, Ian D., and Deborah Cook. "Immunofluorescence techniques." The Journal of Investigative Dermatology 133.1 (2013): e4.
