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Mycoplasma is a member of the family Mycoplasmataceae, and is the smallest known self-replicating pathogen, differing from other genera in that it has no cell wall. Porcine endemic pneumonia (PEP) is a common infectious disease in the pig industry caused by Mycoplasma hyopneumoniae (Mhyo) infection. It has high incidence and low mortality rate, mainly resulting in reduced feed conversion rate and prolonged slaughter time, causing huge economic losses to the global pig industry. Therefore, the establishment of accurate, sensitive and rapid Mhyo detection methods can help to understand the circulation of Mhyo in swine herds, so that corresponding preventive, curative and integrated control measures can be taken, which is crucial to improve Mhyo infection.
Figure 1. M. hyopneumoniae resides intracellularly within PK-15 cells (Raymond, et al.2018).
Mycoplasma hyopneumoniae ELISA Kit Development
The diagnosis of Mhyo infection can be achieved by bacterial isolation, molecular identification and serological testing. Among them, bacterial isolation is considered as the "gold standard" for the diagnosis of Mhyo in pigs, but Mhyo is one of the most difficult mycoplasmas to cultivate. During isolation, Mhyo requires high maintenance of culture medium, long isolation time, low isolation rate, and is often contaminated by Mycoplasma hyorrhea and Mycoplasma flocculents. Although PCR can distinguish Mhyo from Mycoplasma hyorrhea and Mycoplasma flocculents in PEP samples, but this method is too expensive and sample collection can be challenging. ELISA is commonly used to diagnose Mhyo infection in pigs because of its simplicity, easy programming and ability to detect large numbers of samples.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare antibodies specific for surface antigen of Mycoplasma hyopneumoniae, and we can provide you with a range of customized ELISA kits for Mycoplasma hyopneumoniae detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for diagnosis of animal diseases.
The ELISA Kits We Can Develop
| Mhyo SIgA ELISA kit | Mhyo IgG ELISA kit | Mhyo P65 ELISA kit |
| Mhyo Mhp366 ELISA kit | OtherMhyo ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Design and synthesize primers for the nucleic acid sequence encoding the antigenic protein and obtain the target fragment by amplification, connect the target fragment to the expression vector, induce expression to obtain the recombinant protein, and carry out SDS-PAGE and Western blot to identify its immunogenicity, purification recombinant protein. |
| Antibody preparation | Preparation of antibodies specific for surface antigen of Mhyo. |
| ELISA kit development | Optimizing the final reaction conditions, evaluation of sensitivity, specificity and concordance of ELISA kit. |
| ELISAkit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection Mycoplasma hyopneumoniae
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Ready-to-use liquid reagents make assays easy to implement and run
- High specificity & no cross-reactions with other mycoplasmas
- Detect antigens at low levels
- Reliable and rapid screening and detection of seropositive animals
- Each kit is rigorously validated and tested
References
- Tian, Yaqin, et al. "Development of an indirect ELISA for detection of anti-Mycoplasma hyopneumoniae IgG in naturally infected pathogen-induced convalescent sera." BMC Veterinary Research 17.1 (2021): 1-10.
- Raymond, B. B. A., et al. "Mycoplasma hyopneumoniae resides intracellularly within porcine epithelial cells." Scientific Reports 8.1 (2018): 1-13.
- Liu, Maojun, et al. "Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65." Journal of Veterinary Medical Science (2016): 15-0438.
