Services
Ornithobacterium rhinotracheale (ORT) is a slow-growing, highly pleomorphic, non-motile, non-budding Gram-negative short bacillus with chemotactic and mesophilic metabolic properties. Ornitobacteriosis is an acute, highly contagious respiratory infectious disease caused by ORT, which mainly infecting broilers and turkeys. It is clinically characterized by respiratory symptoms, growth failure, and increased mortality. Pathologically, it is characterized by severe unilateral or bilateral fibrinous suppurative pneumonia and airsacculitis. Currently, the disease is prevalent in many developed countries in Europe, America and South Africa, and is on the rise, seriously endangering the development of the poultry industry.
Figure 1. Gram stain of Ornithobacterium rhinotracheale, a: ORT from 48 hrs culture on blood agar, showing short and plump rods; b: bacteria from fluid medium, showing very long rod (Mirzaie, et al.2011).
Ornithobacterium rhinotracheale ELISA Kit Development
Researchers have developed various assays to monitor ORT infection, such as serological methods, bacterial isolation, etc. The advantages of serological testing compared to bacterial isolation are the persistence of antibodies for several weeks after infection and the short time of bacterial shedding. ELISA is a widely used serological method for screening serum samples from chicken flocks. The serotype specificity of ELISA depends on the method of antigen extraction. Boiled extract antigens are serotype specific, whereas extraction of antigens with sodium dodecyl sulfate yields more cross-antigens. According to literature reports, more than 18 serotypes (A-R) of ORT have been identified. Therefore, it is necessary to develop ORT serotype-specific ELISA kits.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare antibodies specific for antigens of ORT, so we can provide you with a range of customized ELISA kits for ORT detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for diagnosis of avian diseases.
The ELISA Kits We Can Develop
| ORT Serotype A-ELISA kit | ORT Serotype B-ELISA kit |
| ORT Serotype E-ELISA kit | ORT Serotype L-ELISA kit |
| ORT Serotype I-ELISA kit | Other ORT ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Preparation of secreted proteins, extraction of ORT antigens by boiling and sodium dodecyl sulfate, preparation of soluble antigens. |
| Antibody preparation | Preparation of antibodies specific for antigens of different serums of ORT, analysis for antibodies specificity and epitope. |
| ELISA kit development | Optimizing the best concentration of ORT antigens and antibody dilution, evaluation of sensitivity, specificity and concordance of ELISA kit. |
| ELISA kit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection Ornithobacterium rhinotracheale
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Highly specificity, low background
- Earlier detection of seroconversion and higher analytical sensitivity
- Each kit is rigorously validated and tested
- Allows high sample throughput
- Reasonable price and short turnaround time
References
- Barbosa, Eunice Ventura, et al. "Ornithobacterium rhinotracheale: An update review about an emerging poultry pathogen." Veterinary Sciences 7.1 (2019): 3.
- Mirzaie, S., et al. "Isolation and characterization of Ornithobacterium rhinotracheale in the commercial turkey, quail flocks and domestic pigeons by bacteriological and molecular methods." (2011): 121-127.
