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Pig production is an important part of global food security, agricultural economy and local and international trade. Infectious diseases affect the health of pigs and the stability and productivity of the global pig industry. Among the most prevalent pathogens associated with porcine diseases are Escherichia coli (causing diarrhea, oedema disease and septicemia), Actinobacillus pleuropneumoniae (causing porcine pleuropneumonia), Streptococcus suis (related to meningitis, arthritis, pneumonia, and septicemia), Bordetella bronchiseptica (involved in atrophic rhinitis and bronchopneumonia) and Staphylococcus hyicus (causing exudative epidermitis). Some novel pig-only pathogens have also been identified, such as the strains of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus, which have resulted in hundreds of millions of dollars of agricultural losses to the world swine industry.
Figure 1. Schematic of the proposed mechanism for porcine epidemic diarrhea virus (PEDV) transportation in swine (Li, et al., 2018).
Porcine Pathogens IFA Kit Development
More and more procine pathogens have been found in recent years, highlighting the emerging or increasing threat of these pathogens to swine health. Immunofluorescence assay (IFA) is a frequently used method for the recognization of characteristic pathogen in pigs and localization of infections on fixed specimens. As a microscopic method, IFA that can detect and visualize the proteins expressed in cells via antigen-antibody reaction. Immunofluorescence can be used to observe how proteins, glycans, and small biological and non-biological antigens are distributed in tissue slices, cultured cell lines, and individual cells. Direct immunofluorescence and Indirect immunofluorescence are few of IFA techniques for detecting porcine infections that have been established.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal pathogens for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. We have a R&D team with extensive experience in veterinary diagnostic and kit development. To support your needs, we can develop a spread of customized immunofluorescence kits for the detection of pig illnesses. Please don't hesitate to contact us, we'll provide you with high-quality products and services.
Porcine Pathogens IFA Kits We Can Develop
| Escherichia coli direct IFA kit | Escherichia coli indirect IFA kit | Streptococcus suis direct IFA kit |
| Streptococcus suis indirect IFA kit | Actinobacillus pleuropneumoniae direct IFA kit | Actinobacillus pleuropneumoniae indirect IFA kit |
| PEDV direct IFA kit | PEDV indirect IFA kit | Other IFA kits you need |
Workflow of Porcine Pathogens IFA Kit Development
| Workflow | Details |
| Sample preparation | Tissue sample or blood sample was taken from the sick pigs. |
| Sample fixation | Incubating the sample for 10 minutes at room temperature in a formalin solution or chilled methanol or acetone to crosslink the proteins. |
| Cell permeabilization | To stain intracellular proteins, permeabilize the cell by incubating in a detergent. |
| Blocking | To minimize intra- or extracellular background signals, block the non-specific antigens by incubating the sample in the serum of the host, in which the secondary antibody was made. |
| Antibody incubation | Primary antibody and secondary antibody were incubated in selected solutions successively. |
| Microscopy | Process the microscopic analysis after counterstain and mounting. |
| Assessment of the assay | Evaluate the assay by digital image analysis and statistical analysis. |
Delivery
- A series of IFA kits for porcine pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High specificity and sensitivity
- The multiple targets detection efficiency
- Reasonable price and short turnaround time
- A large number of samples can be handled simultaneously and stored for certain time
References
- Li, Yuchen, et al., "An alternative pathway of enteric PEDV dissemination from nasal cavity to intestinal mucosa in swine." Nature Communications 9.1 (2018): 1-14.
- Auerbach, J., et al., "Grouping of porcine enteroviruses by indirect immunofluorescence and description of two new serotypes." Journal of Veterinary Medicine, Series B 41.1‐10 (1994): 277-282.
