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Transmissible gastroenteritis virus (TGEV) is a porcine enteric coronavirus belonging to the family Coronaviridae, genus Alphacoronavirus. The genome of TGEV is a positive-stranded RNA molecule, 28.5 kb in length. TGEV spreads via the oronasal route and replicates in the intestinal mucosal epithelial cells and alveolar macrophages of piglets, and subsequently invades the small intestine via the digestive tract or blood. Swine transmissible gastroenteritis (TGE) is a gastrointestinal infectious disease caused by TGEV, and its morbidity and infectivity are relatively high. Once a pig has TGE, it will show symptoms such as vomiting, fever and dehydration, and severe watery diarrhea that can be fatal. In large-scale farming, TGE can cause huge economic loss and seriously endanger the development of pig industry.
Figure 1. Persistent TGEV infection in recovered pigs. Detection of TGEV infection by immunohistochemistry staining in the jejunum. The TGEV antigen stains brown in the cytoplasm of epithelial cells (Xia, Lu, et al. 2018).
Transmissible Gastroenteritis Virus PCR Kit Development
As one of the important diseases hindering the rapid development of the pig industry, TGE has caused huge economic losses to the pig industry. Therefore, developing a rapid and effective method for diagnosing TGEV is crucial for the prevention and control of TGE. Various methods have been developed for the detection of TGEV antigens, including virus isolation, in situ hybridization, immunoelectron microscopy, fluorescence assays, and enzyme-linked immunosorbent assays. However, these methods cannot be used for early detection of TGEV antigens due to the need to detect samples from the intestines of dead pigs and the long detection time. In recent years, various methods based on molecular diagnostic techniques have been developed. RT-PCR is widely used for the detection of TGEV as a convenient and sensitive detection method. However, RT-PCR cannot measure viral load and is less sensitive than real-time PCR. In order to better meet customers' needs for TGEV detection, more PCR methods have been developed, including real-time TaqMan RT-PCR, qRT-PCR, multiplex real-time RT-PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of transmissible gastroenteritis virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Transmissible Gastroenteritis Virus PCR Kits We Can Develop
| TGEV RT-PCR kit | TGEV qRT-PCR kit | TGEV stem-loop RT-PCR kit |
| TGEV multiplex real-time RT-PCR kit | TGEV real-time TaqMan RT-PCR kit | Other TGEV RT-PCR kits |
Workflow of Transmissible Gastroenteritis Virus PCR Kit Development
| Workflow | Details |
| Specimen preparation | Specimens can be collected from pigs that were infected by transmissible gastroenteritis virus. |
| Primer and probes design | Available sequences of transmissible gastroenteritis virus can be retrieved from GenBank and specific primer/probe sets were designed. |
| Nucleic acid extraction | Extract RNA from cell cultures, and RNA concentration, purity and integrity were measured. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of RNA from transmissible gastroenteritis virus. The sensitivity of the PCR kit was evaluated by determining the limit of detection (LoD). |
Delivery
- A series of PCR kits for transmissible gastroenteritis virus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- High sensitivity to detect very small amounts of transmissible gastroenteritis virus in your samples
- Excellent specificity for the detection of transmissible gastroenteritis virus
- Reasonable price and short turnaround time
References
- Xia, Lu, et al. "Impact of TGEV infection on the pig small intestine." Virology Journal 15.1 (2018): 1-7.
- Zhengyu, Y. U., et al. "Development of TaqMan-based real-time PCR assay for detecting transmissible gastroenteritis virus and its application in vaccine evaluation." Agricultural Science & Technology 15.9 (2014).
