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Anaplasma spp. is a group of intracellular bacteria that infect a variety of cell types. Various Anaplasma have been reported, such as Anaplasma marginale, A. bovis, A. centrale, A. ovis, A. platys and A. phagocytophilum. Anaplasma can proliferate in cells and cause anaplasmosis in livestock and wild animals. It is mainly spread through the mouthparts of different species of ticks and other biting mosquitoes. In addition, the transmission of Anaplasma may also occur through needles and other veterinary instruments contaminated with infected blood. During acute Anaplasma infection, symptoms such as fever, anemia, decreased physical condition, decreased milk production and miscarriage may occur. Anaplasma is widespread worldwide, especially in the United States, Europe and some Asian countries, which severely limits livestock production and causes huge agricultural losses.
Figure 1. Field's staining showing Anaplasma spp. infections in representative thin film blood smears (Bargul, et al.2021).
Anaplasma spp. ELISA Kit Development
At present, a variety of anaplasmosis detection methods have been developed, such as indirect fluorescent antibody technology (IFAT), enzyme-linked immunosorbent assay (ELISA), etc. Although the indirect fluorescent antibody technique (IFAT) is one of the most commonly used assays, ELISA has advantages over it because ELISA can obtain results directly from a microplate reader and can evaluate a large number of serum samples. Therefore, ELISA is widely used in the world to regularly monitor the infection of cattle, camels, dogs, goats and other animals with Anaplasma in order to formulate relevant control measures.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare antibodies specific for antigens of Anaplasma spp., so we can provide you with a range of customized ELISA kits for Anaplasma spp. detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for diagnosis of animal diseases.
The ELISA Kits We Can Develop
| Anaplasma marginalecELISA kit | A. bovis cELISA kit | A. phagocytophilum cELISA kit |
| A. centrale cELISA kit | A. platys cELISA kit | A. ovis cELISA kit |
| Anaplasma marginaleiELISA kit | A. bovis iELISA kit | A. phagocytophilum iELISA kit |
| A. platys iELISA kit | A. ovis iELISA kit | A. centrale iELISA kit |
| Anaplasma marginaledasELISA kit | A. bovis dasELISA kit | A. platys dasELISA kit |
| A. phagocytophilum dasELISA kit | A. ovis dasELISA kit | Other Anaplasma spp. ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | DNA extraction, in silico analysis of MSP5, cloning of truncated msp5 gene from Anaplasma spp., protein expression and purification, Western blot for MSP5. |
| Antibody preparation | Preparation of antibodies specific for antigens of Anaplasma spp., analysis for antibodies specificity and epitope. |
| ELISA kit development | Optimizing the best concentration of Anaplasma spp. antigens and antibody dilution, evaluation of sensitivity, specificity and concordance of ELISA kit. |
| ELISA kit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection Anaplasma spp.
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Highly specificity, low background
- Each kit is rigorously validated and tested
- Less equipment and greater flexibility
- Enables differentiation of weak from strong positive samples
- Reasonable price and short turnaround time
References
- Sarli, Macarena, et al. "Development and evaluation of a double-antigen sandwich ELISA to identify Anaplasma marginale–infected and A. centrale–vaccinated cattle." Journal of Veterinary Diagnostic Investigation 32.1 (2020): 70-76.
- Mason, Kathleen L., et al. "Validation of an improved Anaplasma antibody competitive ELISA for detection of Anaplasma ovis antibody in domestic sheep." Journal of Veterinary Diagnostic Investigation 29.5 (2017): 763-766.
- Bargul, Joel L., et al. "Transmission of 'Candidatus Anaplasma camelii'to laboratory animals by camel-specific keds, Hippobosca camelina." BioRxiv (2021).
