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Rotaviruses (RVs) were originally discovered in mice and vervet monkeys and belong to the family Reoviridae, genus Rotavirus. RV is a double-stranded RNA virus with 11 genomic segments encoding 6 structural proteins (VPs) and 6 nonstructural proteins (NSPs). Rotavirus particles have no envelope structure and have an icosahedral symmetry. The rotavirus particles have three types of protein capsids under electron microscopy: nucleocapsid, inner capsid and outer capsid. Rotaviruses are divided into nine species, designated as A-D and F-J, based on nucleotide (nt) sequence differences in the gene encoding the inner coat protein VP6. Among them, rotaviruses of groups A, B, C and H can infect humans and animals, while rotaviruses belonging to groups D, F and G mainly infect animals.
Figure 1. Schematic representation of rotavirus structure. The infectious virion (TLP) has three layers of capsid protein. The innermost core protein VP2 forms an icosahedron with 12 5-fold hubs projecting into the DLP layer. The replication complexes, consisting of VP1 and VP3, are located on the inside of the hubs. Each replication complex is linked to a dedicated dsRNA segment, coiled inside the core. The middle capsid layer protein VP6 is the most conserved and is the basis for demarcation of rotaviruses into different groups. The outermost layer is composed of VP7 glycoprotein with VP4 spike proteins submerged in it (Uprety, Tirth, et al. 2021).
Rotavirus Virus Isolation Kit Development
Rotavirus can infect a variety of animals (such as pigs, cattle, sheep, horses) and cause diarrhea, vomiting, anorexia, dehydration and other symptoms. Rotavirus infection is characterized by a short incubation period, high infectiousness, wide prevalence and high morbidity, and is often mixed with other bacteria and viruses to aggravate the disease, which is one of the main pathogens leading to increased mortality of animal cubs worldwide and causing huge economic losses to the global farming industry. Therefore, it is very important to develop reagents to accurately identify animal rotavirus species for the control and treatment of animal diseases.
BioVenic is a supplier of virus isolation kits and has been working on the development of animal virus diagnostics regent for many years. We have a professional virus isolation technology platform and experienced staff. We can provide you with a series of rotavirus isolation kits to isolate and identify viruses from different animal samples to study their pathogenesis and transmission pathways and to better control the spread of rotavirus among animals. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
The Rotavirus Isolation Kits We Can Develop
Bovine rotavirus isolation kit
Equine rotavirus isolation kit
Goat rotavirus isolation kit
Feline rotavirus isolation kit
Turkey rotavirus isolation kit
Rabbit rotavirus isolation kit
Canine rotavirus isolation kit
Porcine rotavirus isolation kit
Sheep rotavirus isolation kit
Chicken rotavirus isolation kit
Pigeons rotavirus isolation kit
Other rotavirus isolation kit
Workflow of Rotavirus Isolation Kit Development
| Workflow | Details |
|---|---|
| Sample collection | Samples are collected from infected animals. |
| Cell culture | Samples were inoculated onto the culture medium for propagation. |
| RNA isolation and purification | Isolation and purification of rotavirus RNA from cell culture fluid. |
| Structural analysis of rotavirus | The isolated rotavirus acid and protein coat were analyzed. |
| Rotavirus isolation kit development | Development of kit to isolate rotavirus. |
| Rotavirus isolation kit delivery | We will provide you with customized rotavirus isolation kits and complete product quality inspection reports. |
Delivery
- A series of rotavirus isolation kits
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- High sensitivity and reliable results
- Advanced equipment and professional technicians
- Reasonable price and short turnaround time
References
- Dhama, K., et al. "Rotavirus diarrhea in bovines and other domestic animals." Veterinary Research Communications 33.1 (2009): 1-23.
- Uprety, Tirth, Dan Wang, and Feng Li. "Recent advances in rotavirus reverse genetics and its utilization in basic research and vaccine development." Archives of Virology 166.9 (2021): 2369-2386.
