Mouse IgE ELISA Kit

Product Description:

In the assay, the IgE present in samples reacts with the anti-mouse IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-mouse IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of mouse IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.

Antibody Type:

ELISA

Reactivity:

Murine/Mouse

Host:

Goat

Specificity/Target:

IgE

Detection Range:

6.25 ng/ml - 400 ng/ml

Sensitivity:

1.911 ng/ml

Assay Time:

70 minutes

Sample Type:

Plasma, serum

Storage Conditions:

2-8°C