Dog IgG ELISA Kit

Product Description:

In the assay, the IgG present in samples reacts with the anti-dog IgG Fc antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-dog IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of dog IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolataed from the standard curve constructed from the standards, and corrected for sample dilution.

Antibody Type:

ELISA

Reactivity:

Canine/Dog

Host:

Goat

Specificity/Target:

IgG h+l

Detection Range:

25 ng/ml - 800 ng/ml

Sensitivity:

7.148 ng/ml

Assay Time:

40 minutes

Sample Type:

Plasma, serum

Storage Conditions:

2-8°C