Cat IgG ELISA Kit
Products Name
Cat IgG ELISA Kit
Cat.No.
IVEK0017
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Product Description:
The cat IgG ELISA kit is intended for the quantitative determination of total cat IgG in biological samples.
In the assay, the IgG present in samples reacts with the anti-cat IgG Fc antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-cat IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of cat IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
In the assay, the IgG present in samples reacts with the anti-cat IgG Fc antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-cat IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of cat IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
Antibody Type:
ELISA
Reactivity:
Feline/Cat
Host:
Rabbit
Specificity/Target:
IgG h+l
Detection Range:
12.5 ng/ml - 800 ng/ml
Sensitivity:
4.095 ng/ml
Assay Time:
80 minutes
Sample Type:
Plasma, serum
Storage Conditions:
2-8°C
