Originally defined as molecules present on the cell surface of leukocytes and recognized by specific antibody molecules, CD antigens now also include some intracellular molecules and molecules present on cells other than leukocytes. Since 1992, eight meetings of the International Leukocyte Differentiation Antigen Collaborative Group have been held, and the membrane molecules recognized by monoclonal antibodies from different laboratories have been unified and named as CD antigens. The nomenclature is as follows.
i) Two or more monoclonal antibodies that recognize the same antigen are used as an antibody group, and each antibody group is represented by a number, such as CD3, CD20, etc.
ii) When different antigenic determinants exist on the same CD and can be recognized by specific monoclonal antibodies, the CD numbers are followed by lowercase letters to distinguish them, such as CD1 can be divided into CD1a, CD1b, CD1c and CD1d.
iii) When CD numbers are followed by a capital letter "R", it means that the CD is only found on a specific type of cell, e.g. CD45 is a common antigen of leukocytes, while CD45R is a membrane antigen found only on monocytes, macrophages, B lymphocytes and some T lymphocytes.
Figure 1. B cell CD antigen.
Bovine CD Marker Antibodies
CD molecules are commonly used as cell markers for the identification and isolation of leukocyte populations, subpopulations and differentiation stages. The following table shows several common bovine CD markers. Monoclonal antibodies against these molecules have proven essential in biomedical research and diagnostics as well as in biotechnology.
| Type | Description |
| CD4 / CD8 | For the study of T lymphocytes in flow cytometry, immunohistochemistry, and immunoprecipitation. |
| CD63 | Expressed on activated platelets, monocytes and dendritic cell subsets in dairy cows. |
| CD172a | Expressed on a subset of monocytes and afferent lymphoid shroud cells. |
| CD25 | A ~55 kDa glycoprotein also known as Interleukin-2 receptor alpha chain, expressed by activated T cells. |
| CD40 | A 260 amino acid single pass type 1 transmembrane glycoprotein, expressed by B lymphocytes and a subset of T lymphocytes. |
| CD335 | A type I transmembrane protein, expressed on bovine NK cells. |
BioVenic is a company dedicated to researching veterinary antibodies. We have a professional antibody development platform and research team who have extensive experience in veterinary diagnostics and kit development, and we can offer you a variety of antibodies against bovine CD markers. These antibodies are primarily used to study the bovine adaptive and innate immune system and help to elucidate the function of T cells, B cells, dendritic cells and macrophages. If you have any needs, please feel free to contact us. We will provide you with high-quality products according to your needs.
