Amyloid Beta (1-42) ELISA Kit (Rat/Mouse)

Product Overview:

Rat/mouse amyloid beta (1-42) ELISA kit can be used for detecting quantitative concentrations of amyloid beta (Aβ, Abeta, beta-amyloid)(1-42) in rat and mouse EDTA plasma or serum samples. This assay has a minimum sensitivity detection limit of 0.05 pg/ml.

Product Description:

Amyloid beta (also known as beta-amyloid, Aβ and Abeta) is made up of peptides that range from 36-43 amino acids and these are important in Alzheimer’s disease because amyloid plaques have been discovered in the brains of Alzheimer patients. Senile plaques that have been identified consist of both Aβ40 and Aβ42, whereas the vascular amyloid predominately contains the shorter Aβ40. Also, due to its more hydrophobic nature Aβ42 is regarded as the more amyloidogenic form of the peptide. Many functions of amyloid beta have been found such as activation of kinase enzymes, regulation of cholesterol transport, functioning as a transcription factor, protection against oxidative stress and anti-microbial activity.

Product Content:

Microtiter plate: coated with anti-amyloid beta (38-42).
Enzyme conjugate concentrate (30x concentrated).
Standard contains: mouse/rat Aβ (1-42).
Assay buffer.
Conjugate buffer.
TMB substrate solution.
Stop solution.
Wash buffer (40x).

Reactivity:

Rat, mouse

Specificity/Target:

Rat amyloid beta (1-42), mouse amyloid beta (1-42), rat Aβ (1-42), mouse Aβ (1-42)

Detection Range:

1.56 – 100.0 pg/ml

Sensitivity:

The minimum sensitivity detection limit of beta-amyloid (Aβ, Abeta) using current rat/mouse amyloid beta (1-42) ELISA kit was approximately 0.05 pg/ml. The dynamic range for this assay is 1.56 – 100.0 pg/ml.

Assay Characteristics:

Rat, mouse amyloid beta (1-42): ELISA.
Sensitivity: 0.05 pg/mL.
Specificity: mouse/rat Aβ (1-42)(100%), human Aβ (1-42)(1.3 %) and mouse/rat Aβ (1-40)(<0.1 % ).
Intra assay: 3.4 – 5.8%.
Inter assay: 2.8 – 8.0%.

Sample Type:

Plasma, serum

Storage Conditions:

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.