Angiopoietin-like 3 (ANGPTL3) ELISA Kit (Mouse)

Product Overview:

Mouse ANGPTL3 ELISA kit is designed for analysing quantitative amounts of angiopoietin-like 3 (ANGPTL3) in mouse serum, cell culture media and plasma samples. This assay has a minimum sensitivity detection limit of 0.04 ng/ml.

Product Description:

Angiopoietin-like 3 (ANGPTL3) is a protein that belongs to the angiopoietin-like family of secreted factors which is expressed mainly in the liver. It contains the standard characteristic structure that can be found in angiopoietins, a C-terminal fibrinogen (FBN)-like domain, N-terminal coiled-coil domain and a signal peptide. Some of the functions of ANGPTL3 include acting as a dual inhibitor of endothelial lipase (EL) and lipoprotein lipase (LPL), in order to increase the levels of HDL cholesterol and plasma triglyceride in rodents. It has been found to play a major role in promoting the uptake of circulating triglycerides into white adipose tissue during the fed state.

Product Content:

Pre-coated plate: anti-mouse angiopoietin-like 3 (ANGPTL3).
Labeled antibody (30x Conc.).
Standard: recombinant mouse angiopoietin-like 3.
EIA buffer.
Solution for labeled antibody.
TMB chromogen.
Stop solution.
Wash buffer concentrated.

Reactivity:

Mouse

Specificity/Target:

Mouse ANGPTL3, mouse angiopoietin-like 3

Detection Range:

0.31 – 20.0 ng/ml

Sensitivity:

The minimum sensitivity detection limit of angiopoietin-like 3 using current mouse ANGPTL3 ELISA kit was approximately 0.04 ng/ml. The dynamic range for this assay is 0.31 – 20.0 ng/ml.

Assay Characteristics:

Mouse ANGPTL3: ELISA.
Titer assay: 90.4 – 126.5%.
Added recovery assay: 99.2 – 109.9%.
Intra – assay: 3.6 – 6.6%.
Inter – assay: 6.5 – 7.0%.

Sample Type:

Plasma, cell culture media, serum

Storage Conditions:

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.