Angiotensinogen ELISA Kit (Rat)

Product Overview:

Rat angiotensinogen ELISA kit can be used for measuring quantitative levels of angiotensinogen (AGT) in rat plasma, serum, cell culture supernatant and urine samples. This assay has a minimum sensitivity detection limit of 0.02 ng/ml.

Product Description:

Angiotensinogen (AGT) is an important member of the serpin family of proteins, it is an alpha-2-globulin that is constitutively produced and released into circulation by the liver. Predominant function involves regulating fluid balance and blood pressure through the renin-angiotensin system. The concentration of plasma angiotensinogen can be elevated by increasing plasma estrogen, corticosteroid, angiontensin II and thyroid hormone levels. Angiotensinogen is catalytically cleaved by renin to generate angiotensin I under conditions of low blood pressure. There is evidence to indicate that it can be associated with heterogeneous disorders, predisposition to hypertension, pre-eclampsia, morbidity, neonatal or foetal mortality.

Product Content:

Pre-coated plate: anti-rat AGT (135).
HRP labeled antibody (30x Conc.).
Standard: recombinant rat angiotensinogen.
EIA buffer.
Solution for labeled antibody.
TMB chromogen.
Stop solution.
Wash buffer (40x Conc.).

Reactivity:

Rat

Specificity/Target:

Rat angiotensinogen, rat AGT

Detection Range:

0.31 – 20.0 ng/ml

Sensitivity:

The minimum sensitivity detection limit of AGT using current rat angiotensinogen ELISA kit was approximately 0.02 ng/ml. The dynamic range for this assay is 0.31 – 20.0 ng/ml.

Assay Characteristics:

Rat angiotensinogen: ELISA.
Titer assay: 92.7 – 104.5%.
Added recovery assay: 82.4 – 99.5%.
Intra – assay: 3.3 – 10%.
Inter – assay: 8.5 – 15.8%.
Specificity: 100%.

Sample Type:

Cell culture supernatant, serum, plasma, urine

Storage Conditions:

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.