Overview

Description:

Anti canine CD3/CD8 dual color reagent is a dual colour reagent recognising canine CD3 and CD8 cell surface antigens. Clone CA17.2A12 recognises canine CD3, a T cell differentiation antigen expressed on mature T lymphocytes.

Clone YCATE 55.9 recognises canine CD8, which is expressed by cytotoxic T cells. YCATE 55.9 binds to CD8 alpha.

Product Form:

Dual colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized.

Reconstitution:

Reconstitute with 1.0 ml distilled water.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.

Antibody Isotypes:

FITC reagent: IgG1 (MOUSE).
RPE reagent: IgG1 (RAT).

Excitation Max (nm):

FITC: 490.
RPE 488 nm laser: 496.
RPE 561nm laser: 546.

Emission Max (nm):

FITC: 525.
RPE 488 nm laser: 578.
RPE 561nm laser: 578.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.

Storage:

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.

Note:

DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Product Type:

Dual color reagent

Clone:

CA17.2A12/YCATE55.9

Isotype:

Cocktail

Specificity:

CD3/CD8

Target:

Dog

Anti Dog CD3:FITC/CD8:RPE

Overview

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