Bovine Haptoglobin ELISA Assay

Product Overview:

The bovine haptoglobin ELISA assay kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring haptoglobin in biological samples of cattle. The bovine haptoglobin kit does not cross-react with sheep haptoglobin.

Product Description:

Acute phase proteins are plasma proteins that increase in concentration following infection, inflammation or trauma. Haptoglobin (Hp) is a heterogeneous plasma protein mainly synthesized by the liver. The haptoglobin monomer consists of two heavy chains, beta (40 kD) and two light chains, alpha, alpha 1 (9 kD) and alpha 2 (16 kD), which are linked by disulfide bonds. The three main haptoglobin types are: Hp1-1 is monomeric (98kD), Hp1-2 is polymeric at about 200 kD, and Hp2-2 is polymeric at about 400 kD.

Sensitivity:

6.258 ng/ml

Dynamic Range:

15.6 ng/ml- 1000 ng/ml

Incubation Time:

40 minutes

Sample Type:

Plasma, serum

Sample Size:

100 µl

Specimen Collection and Handling:

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.