Bovine IgM ELISA Assay

Product Overview:

The bovine IgM ELISA assay kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgM in bovine biological samples.

Assay Principle:

In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of bovine IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of bovine IgM in the test sample. The quantity of bovine IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Sensitivity:

3.006 ng/ml

Dynamic Range:

12.5 ng/ml – 400 ng/ml

Incubation Time:

80 minutes

Sample Type:

Plasma, serum

Sample Size:

100 µl

Specimen Collection and Handling:

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.