Canine CRP ELISA Assay

Product Overview:

The canine CRP ELISA assay kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CRP in dog biological samples.

Assay Principle:

In this assay the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of c-reactive protein in the sample. The quantity of c-reactive protein in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Sensitivity:

0.855 ng/ml

Dynamic Range:

3.125 ng/ml – 200 ng/ml

Incubation Time:

25 minutes

Sample Type:

Biological fluids

Sample Size:

100 µl

Specimen Collection and Handling:

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.