Canine IgE ELISA Assay

Product Overview:

The canine IgE ELISA assay kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in serum or plasma of dogs.

Assay Principle:

In this assay the dog IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the tested sample; thus, the absorbance, at 450 nm, is a measure of the concentration of canine IgE in the sample. The quantity of dog IgE can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Sensitivity:

1.728 ng/ml

Dynamic Range:

6.25 ng/ml – 400 ng/ml

Incubation Time:

70 minutes

Sample Type:

Plasma, serum

Sample Size:

100 µl

Specimen Collection and Handling:

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.