Dog Thymidine Kinase (TK1) ELISA Assay Kit

Product Overview:

The dog thymidine kinase ELISA is a highly sensitive two-site enzyme linked immunoassay for measuring TK1 in dog biological samples. The canine thymidine kinase ELISA is intended for research use only and not for use in diagnostic or clinical procedures.

Assay Principle:

In this assay the thymidine kinase (TK1) present in samples reacts with the anti-TK1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-TK1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of TK1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of TK1 in the test sample. The quantity of TK1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Sensitivity:

0.094 ng/ml

Dynamic Range:

0.078 ng/ml – 5.0 ng/ml

Incubation Time:

2 hrs 30 mins

Sample Type:

Plasma, serum

Sample Size:

100 µl

Specimen Collection and Handling:

All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions when handling and disposing.
If blood samples are clotted, grossly hemolyzed, lipemic, or the integrity of the sample is of concern, make a note and interpret results with caution.