Goat anti Rabbit IgG (H/L):FITC
Products Name
Goat anti Rabbit IgG (H/L):FITC
Cat.No.
RRIA0013
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Overview
Description:
Goat anti rabbit IgG (H/L) antibody recognises rabbit IgG heavy and light chains and the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry.
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Antisera to rabbit IgG (H+L) were raised by repeated immunisations of goats with normal rabbit IgG. Purified IgG was prepared from whole serum by affinity chromatography on pooled rabbit IgG covalently linked to agarose. The preparation has been cross absorbed against human and mouse sera and purified paraproteins to minimise potential cross reactivity.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
< 0.1% Sodium azide (NaN3).
Immunogen:
Normal rabbit IgG.
Approx. Protein Concentrations:
IgG concentration 1.0 mg/ml.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
Guaranteed until date of expiry. Please see product label.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Product Type:
Polyclonal antibody
Isotype:
Polyclonal IgG
Specificity:
IgG
Region:
H/L
Target:
Rabbit
