Guinea Pig IgA ELISA

Product Overview:

The guinea pig IgA ELISA assay kit is a highly sensitive two-site enzyme immunoassay for measuring IgA in biological samples of guinea pigs.

Assay Principle:

In this guinea pig IgA assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Sensitivity:

1.212 ng/ml

Dynamic Range:

6.25 ng/ml – 200 ng/ml

Incubation Time:

50 minutes

Sample Type:

Plasma, serum

Sample Size:

100 µl

Specimen Collection and Handling:

Blood should be collected by venipuncture and the serum separated from the cells, after clot formation, by centrifugation. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Specimens may be shipped at room temperature and then stored refrigerated at 2-8°C if testing is to take place within one week after collection. If testing is to take place later than one week, specimens should be stored at -20°C. Avoid repeated freeze-thaw cycles.