Hamster PLBL2 ELISA Assay

Product Overview:

The hamster PLBL2 ELISA assay kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring PLBL2 as a host cell impurity protein in cultures derived from Chinese hamster ovary cells.

Assay Principle:

In this assay the PLBL2 present in samples reacts with the anti-PLBL2 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-PLBL2 is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. Following another washing step, the complexes are assayed by the addition of a chromo-genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of PLBL2 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of PLBL2 in the test sample. The quantity of PLBL2 can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Dynamic Range:

20 ng/mL – 0.3125 ng/ml

Incubation Time:

170 minutes

Sample Type:

Tissue culture fluids

Sample Size:

100 µl

Specimen Collection and Handling:

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles.