Horse CRP ELISA Assay

Product Overview:

The horse CRP ELISA assay kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CRP in horse biological samples.

Product Description:

Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. The first acute phase protein to be recognized was discovered in humans by Tillet and Frances in 1930. This C-reactive protein (CRP) is so named because it is able to effect precipitation of somatic C-polysaccharide of Streptococcus pneumonia. CRP is an alpha globulin with a mass of 110,000 to 140,000 daltons, and composed of five identical subunits, which are non-covalently assembled as a cyclic pentamer.

Sensitivity:

1.198 ng/ml

Dynamic Range:

3.13 ng/ml – 200 ng/ml

Incubation Time:

70 minutes

Sample Type:

Plasma, serum

Sample Size:

100 µl

Specimen Collection and Handling:

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.